RRC ID 72518
Author Miettinen TP, Kang JH, Yang LF, Manalis SR.
Title Mammalian cell growth dynamics in mitosis.
Journal Elife
Abstract The extent and dynamics of animal cell biomass accumulation during mitosis are unknown, primarily because growth has not been quantified with sufficient precision and temporal resolution. Using the suspended microchannel resonator and protein synthesis assays, we quantify mass accumulation and translation rates between mitotic stages on a single-cell level. For various animal cell types, growth rates in prophase are commensurate with or higher than interphase growth rates. Growth is only stopped as cells approach metaphase-to-anaphase transition and growth resumes in late cytokinesis. Mitotic arrests stop growth independently of arresting mechanism. For mouse lymphoblast cells, growth in prophase is promoted by CDK1 through increased phosphorylation of 4E-BP1 and cap-dependent protein synthesis. Inhibition of CDK1-driven mitotic translation reduces daughter cell growth. Overall, our measurements counter the traditional dogma that growth during mitosis is negligible and provide insight into antimitotic cancer chemotherapies.
Volume 8
Published 2019-5-7
DOI 10.7554/eLife.44700
PII 44700
PMID 31063131
PMC PMC6534395
MeSH Animals Biomass Cell Line Cell Proliferation* Chickens Humans Mice Mitosis* Protein Biosynthesis Single-Cell Analysis
IF 7.08
Resource
Human and Animal Cells Ba/F3(RCB4476)