論文 - 詳細
| RRC ID | 72518 |
|---|---|
| 著者 | Miettinen TP, Kang JH, Yang LF, Manalis SR. |
| タイトル | Mammalian cell growth dynamics in mitosis. |
| ジャーナル | Elife |
| Abstract |
The extent and dynamics of animal cell biomass accumulation during mitosis are unknown, primarily because growth has not been quantified with sufficient precision and temporal resolution. Using the suspended microchannel resonator and protein synthesis assays, we quantify mass accumulation and translation rates between mitotic stages on a single-cell level. For various animal cell types, growth rates in prophase are commensurate with or higher than interphase growth rates. Growth is only stopped as cells approach metaphase-to-anaphase transition and growth resumes in late cytokinesis. Mitotic arrests stop growth independently of arresting mechanism. For mouse lymphoblast cells, growth in prophase is promoted by CDK1 through increased phosphorylation of 4E-BP1 and cap-dependent protein synthesis. Inhibition of CDK1-driven mitotic translation reduces daughter cell growth. Overall, our measurements counter the traditional dogma that growth during mitosis is negligible and provide insight into antimitotic cancer chemotherapies. |
| 巻・号 | 8 |
| 公開日 | 2019-5-7 |
| DOI | 10.7554/eLife.44700 |
| PII | 44700 |
| PMID | 31063131 |
| PMC | PMC6534395 |
| MeSH | Animals Biomass Cell Line Cell Proliferation* Chickens Humans Mice Mitosis* Protein Biosynthesis Single-Cell Analysis |
| IF | 7.08 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 7 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Ba/F3(RCB4476) |