RRC ID 72519
著者 Ishizuka T, Goshima H, Ozawa A, Watanabe Y.
タイトル β1-adrenoceptor stimulation enhances the differentiation of mouse induced pluripotent stem cells into neural progenitor cells.
ジャーナル Neurosci Lett
Abstract The cyclic AMP/protein kinase A signaling pathway is thought to be involved in neural differentiation of mesenchymal stem cells. In the present study, we examined the involvement of β-adrenoceptor signaling on the differentiation of mouse induced pluripotent stem (iPS) cells into neural progenitor cells. Mouse iPS cells were cultured on ultra-low-attachment dishes to induce embryoid body (EB) formation. All-trans retinoic acid (ATRA, 1 μM) and/or the β-adrenoceptor agonist l-isoproterenol (0.3 or 1 μM) were added to the EB cultures for 4 days, then EBs were plated on gelatin-coated plates and cultured for 7 or 14 days. Subtype-specific antibody staining revealed that mouse iPS cells express β(1)-adrenoceptors predominantly. Although treatment with l-isoproterenol alone did not affect the expression of Nestin (a specific marker for neural progenitor cells), l-isoproterenol significantly enhanced ATRA-induced Nestin expression. Pretreatment of EBs with either atenolol (a selective β(1)-adrenoceptor antagonist) or H89 (a protein kinase A inhibitor) significantly inhibited the l-isoproterenol-enhancement of ATRA-induced Nestin expression. In addition, the l-isoproterenol treatment significantly enhanced ATRA-induced expression of NeuN (a neuron-specific nuclear protein). These findings suggest that β(1)-adrenoceptor stimulation enhances ATRA-induced neural differentiation of mouse iPS cells.
巻・号 525(1)
ページ 60-5
公開日 2012-9-6
DOI 10.1016/j.neulet.2012.07.028
PII S0304-3940(12)00965-2
PMID 22828480
MeSH Adrenergic beta-1 Receptor Agonists / pharmacology* Animals Cell Differentiation / drug effects Cell Line Cyclic AMP-Dependent Protein Kinases / metabolism Embryoid Bodies / cytology Fluorescent Antibody Technique Induced Pluripotent Stem Cells / cytology Induced Pluripotent Stem Cells / drug effects* Induced Pluripotent Stem Cells / metabolism Intermediate Filament Proteins / metabolism Isoproterenol / pharmacology* Mice Nerve Tissue Proteins / metabolism Nestin Neural Stem Cells / cytology Neural Stem Cells / drug effects* Receptors, Adrenergic, beta-1 / metabolism*
IF 2.274
リソース情報
ヒト・動物細胞 iPS-MEF-Ng-20D-17(APS0001)