RRC ID 72584
Author Hayashi S, Mikami T, Murai Y, Takano Y, Imura J.
Title Α-tubulin nuclear overexpression is an indicator of poor prognosis in patients with non-Hodgkin's lymphoma.
Journal Int J Mol Med
Abstract In the present study, the newly established mouse monoclonal antibody, Y-49, binding to a specific epitope of α-tubulin, was used to examine immunohistochemical reactivity in 116 patients with non-Hodgkin's lymphoma (NHL). The protein was detected at elevated levels in the nuclei of human proliferating cells by western blot analysis, flow cytometry and immunohistochemical analysis. The relatively weak binding in the cytoplasm was evident in almost all cases. The investigation of the correlation between immuno-histochemical positivity and clinicopathological variables revealed links with the MIB-1 proliferation index and poor survival. Nuclear positivity with Y-49 was more frequent in older-aged patients, those with nodal NHL and in those who harbored the diffuse large B-cell histological subtype, and was strongly associated with high MIB-1 labeling indices (LIs). Survival analysis by the Kaplan-Meier method revealed statistically significant differences between patients with high and low Y-49 LIs (p=0.0181), even in the group with advanced (stage III/IV) disease (p=0.0327). Multivariate analysis revealed that overexpression of α-tubulin is an independent prognostic factor in NHL with a relative risk of 2.786.
Volume 34(2)
Pages 483-90
Published 2014-8-1
DOI 10.3892/ijmm.2014.1793
PMID 24898903
MeSH Adolescent Adult Aged Aged, 80 and over Animals Antibodies, Monoclonal / immunology Antibodies, Monoclonal / metabolism* Cell Proliferation / genetics Child Female Flow Cytometry Gene Expression Regulation, Leukemic / immunology Gene Expression Regulation, Neoplastic Humans Inhibitor of Apoptosis Proteins / immunology Kaplan-Meier Estimate Lymphoma, Non-Hodgkin / immunology Lymphoma, Non-Hodgkin / metabolism* Lymphoma, Non-Hodgkin / pathology Male Mice Middle Aged Neoplasm Staging Prognosis* Repressor Proteins / immunology Survivin Tubulin / biosynthesis* Tubulin / immunology
IF 2.928
Human and Animal Cells Raji MKN28