RRC ID 72591
著者 Furumoto T, Ozawa N, Inami Y, Toyoshima M, Fujita K, Zaiki K, Sahara S, Akita M, Kitamura K, Nakaoji K, Hamada K, Tamai K, Kaneda Y, Maeda A.
タイトル Mallotus philippinensis bark extracts promote preferential migration of mesenchymal stem cells and improve wound healing in mice.
ジャーナル Phytomedicine
Abstract In the present study, we report the effects of the ethanol extract from Mallotus philippinensis bark (EMPB) on mesenchymal stem cell (MSC) proliferation, migration, and wound healing in vitro and in a mouse model. Chemotaxis assays demonstrated that EMPB acted an MSC chemoattractant and that the main chemotactic activity of EMPB may be due to the effects of cinnamtannin B-1. Flow cytometric analysis of peripheral blood mononuclear cells in EMPB-injected mice indicated that EMPB enhanced the mobilization of endogenous MSCs into blood circulation. Bioluminescent whole-animal imaging of luciferase-expressing MSCs revealed that EMPB augmented the homing of MSCs to wounds. In addition, the efficacy of EMPB on migration of MSCs was higher than that of other skin cell types, and EMPB treatment improved of wound healing in a diabetic mouse model. The histopathological characteristics demonstrated that the effects of EMPB treatment resembled MSC-induced tissue repair. Taken together, these results suggested that EMPB activated the mobilization and homing of MSCs to wounds and that enhancement of MSC migration may improve wound healing.
巻・号 21(3)
ページ 247-53
公開日 2014-2-15
DOI 10.1016/j.phymed.2013.09.003
PII S0944-7113(13)00360-7
PMID 24182990
MeSH Animals Chemotactic Factors Chemotaxis / drug effects* Diabetes Complications / drug therapy* Diabetes Mellitus, Experimental / complications Disease Models, Animal Leukocytes, Mononuclear / metabolism Mallotus Plant / chemistry* Mesenchymal Stem Cells / drug effects* Mice Phytotherapy* Plant Bark Plant Extracts / pharmacology Plant Extracts / therapeutic use Proanthocyanidins / pharmacology Proanthocyanidins / therapeutic use Wound Healing / drug effects* Wounds and Injuries / drug therapy*
IF 4.268
リソース情報
ヒト・動物細胞 KUM6(RCB2113)