| RRC ID |
72594
|
| Author |
Hayashi KI, Arai K, Aoi Y, Tanaka Y, Hira H, Guo R, Hu Y, Ge C, Zhao Y, Kasahara H, Fukui K.
|
| Title |
The main oxidative inactivation pathway of the plant hormone auxin.
|
| Journal |
Nat Commun
|
| Abstract |
Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated in auxin inactivation. In this study, we show that the predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via the GH3-ILR1-DAO pathway. IAA is first converted to IAA-amino acid conjugates by GH3 IAA-amidosynthetases. The IAA-amino acid conjugates IAA-aspartate (IAA-Asp) and IAA-glutamate (IAA-Glu) are storage forms of IAA and can be converted back to IAA by ILR1/ILL amidohydrolases. We further show that DAO1 dioxygenase irreversibly oxidizes IAA-Asp and IAA-Glu into 2-oxindole-3-acetic acid-aspartate (oxIAA-Asp) and oxIAA-Glu, which are subsequently hydrolyzed by ILR1 to release inactive oxIAA. This work established a complete pathway for the oxidative inactivation of auxin and defines the roles played by auxin homeostasis in plant development.
|
| Volume |
12(1)
|
| Pages |
6752
|
| Published |
2021-11-22
|
| DOI |
10.1038/s41467-021-27020-1
|
| PII |
10.1038/s41467-021-27020-1
|
| PMID |
34811366
|
| PMC |
PMC8608799
|
| MeSH |
Amidohydrolases
Amino Acids
Arabidopsis / genetics
Arabidopsis / metabolism
Arabidopsis Proteins
Aspartic Acid
Dioxygenases
Gene Expression Regulation, Plant
Glutamic Acid
Homeostasis
Hydrolysis
Indoleacetic Acids / metabolism*
Oxidation-Reduction
Oxidative Stress
Oxindoles / metabolism
Plant Development
Plant Growth Regulators / genetics
Plant Growth Regulators / metabolism*
Signal Transduction
|
| IF |
12.121
|
| Resource |
| Arabidopsis / Cultured plant cells, genes |
pda02504
pda11310 |
