Reference - Detail
| RRC ID | 72594 |
|---|---|
| Author | Hayashi KI, Arai K, Aoi Y, Tanaka Y, Hira H, Guo R, Hu Y, Ge C, Zhao Y, Kasahara H, Fukui K. |
| Title | The main oxidative inactivation pathway of the plant hormone auxin. |
| Journal | Nat Commun |
| Abstract |
Inactivation of the phytohormone auxin plays important roles in plant development, and several enzymes have been implicated in auxin inactivation. In this study, we show that the predominant natural auxin, indole-3-acetic acid (IAA), is mainly inactivated via the GH3-ILR1-DAO pathway. IAA is first converted to IAA-amino acid conjugates by GH3 IAA-amidosynthetases. The IAA-amino acid conjugates IAA-aspartate (IAA-Asp) and IAA-glutamate (IAA-Glu) are storage forms of IAA and can be converted back to IAA by ILR1/ILL amidohydrolases. We further show that DAO1 dioxygenase irreversibly oxidizes IAA-Asp and IAA-Glu into 2-oxindole-3-acetic acid-aspartate (oxIAA-Asp) and oxIAA-Glu, which are subsequently hydrolyzed by ILR1 to release inactive oxIAA. This work established a complete pathway for the oxidative inactivation of auxin and defines the roles played by auxin homeostasis in plant development. |
| Volume | 12(1) |
| Pages | 6752 |
| Published | 2021-11-22 |
| DOI | 10.1038/s41467-021-27020-1 |
| PII | 10.1038/s41467-021-27020-1 |
| PMID | 34811366 |
| PMC | PMC8608799 |
| MeSH | Amidohydrolases Amino Acids Arabidopsis / genetics Arabidopsis / metabolism Arabidopsis Proteins Aspartic Acid Dioxygenases Gene Expression Regulation, Plant Glutamic Acid Homeostasis Hydrolysis Indoleacetic Acids / metabolism* Oxidation-Reduction Oxidative Stress Oxindoles / metabolism Plant Development Plant Growth Regulators / genetics Plant Growth Regulators / metabolism* Signal Transduction |
| IF | 12.121 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 54 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| Arabidopsis / Cultured plant cells, genes | pda02504 pda11310 |