論文 - 詳細
| RRC ID | 72599 |
|---|---|
| 著者 | Haga E, Endo Y, Haruta M, Koba C, Matsumura K, Takamatsu K, Ikeda T, Nishimura Y, Senju S. |
| タイトル | Therapy of peritoneally disseminated colon cancer by TAP-deficient embryonic stem cell-derived macrophages in allogeneic recipients. |
| ジャーナル | J Immunol |
| Abstract |
We established a method to generate a large quantity of myeloid lineage cells from mouse embryonic stem (ES) cells, termed ES cell-derived proliferating myeloid cell lines (ES-ML). ES-ML continuously proliferated in the presence of M-CSF and GM-CSF. ES-ML genetically modified to express an anti-HER2 (neu) mAb single-chain V region fragment reduced the number of cocultured mouse Colon-26 cancer cells expressing HER2. Stimulation of ES-ML with IFN-γ plus LPS or TNF resulted in almost complete killing of the Colon-26 cells by the ES-ML, and the cytotoxicity was mediated, in part, by NO produced by ES-ML. When ES-ML were injected into mice with i.p. established Colon-26 tumors, they efficiently infiltrated the tumor tissues. Injection of ES-ML with rIFN-γ and LPS inhibited cancer progression in the mouse peritoneal cavity. Coinjection of TNF-transfected or untransfected ES-ML with rIFN-γ inhibited cancer growth and resulted in prolonged survival of the treated mice. In this experiment, transporter associated with Ag processing (TAP)1-deficient ES-ML exhibited therapeutic activity in MHC-mismatched allogeneic recipient mice. Despite the proliferative capacity of ES-ML, malignancy never developed from the transferred ES-ML in the recipient mice. In summary, TAP-deficient ES-ML with anticancer properties exhibited a therapeutic effect in allogeneic recipients, suggesting the possible use of TAP-deficient human-induced pluripotent stem cell-derived proliferating myeloid cell lines in cancer therapy. |
| 巻・号 | 193(4) |
| ページ | 2024-33 |
| 公開日 | 2014-8-15 |
| DOI | 10.4049/jimmunol.1303473 |
| PII | jimmunol.1303473 |
| PMID | 25031460 |
| MeSH | ATP-Binding Cassette Transporters / genetics* Animals Antibodies, Monoclonal / immunology Cell Differentiation / immunology Cell Line, Tumor Colonic Neoplasms / immunology Colonic Neoplasms / therapy* Cytotoxicity, Immunologic Embryonic Stem Cells / immunology* Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology Interferon-gamma / pharmacology Lipopolysaccharides Macrophages / immunology* Mice Mice, Inbred BALB C Mice, Inbred C57BL Pluripotent Stem Cells / immunology Receptor, ErbB-2 / immunology Single-Chain Antibodies / immunology Transplantation, Homologous |
| IF | 4.886 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Colon-26(RCB2657) |