RRC ID 72600
Author Yang D, Li T, Xu M, Gao F, Yang J, Yang Z, Le W.
Title Graphene oxide promotes the differentiation of mouse embryonic stem cells to dopamine neurons.
Journal Nanomedicine (Lond)
Abstract AIM:Nanoparticles are easier to pass through cell membranes, and they are considered to be the ideal biocompatible and mechanically stable platforms for supporting stem cell growth and differentiation. The aim of this study is to determine the effects of carbon nanotubes (CNTs), graphene oxide (GO) and graphene (GR) on the dopamine neural differentiation of mouse embryonic stem cells (ESCs).
MATERIALS & METHODS:GO was prepared according to a modified Hummers method. GR was synthesized by reduction of GO via L-ascorbic acid as a reductant in an aqueous solution at room temperature. CNTs were fabricated by chemical vapor deposition method. ESCs were differentiated by a stromal cell-derived inducing activity (SDIA) method after 10 days coculture with PA6 cells. The dopamine neural differentiation of the ESCs-GFP was examined by immunocytochemistry and real-time PCR.
RESULTS:We found that only GO could effectively promote dopamine neuron differentiation after induction of SDIA and further enhance dopamine neuron-related gene expression compared with cells treated with no nanoparticle control, and the other two nanoparticles (CNTs and GR).
CONCLUSION:These findings suggest that GO is a promising nanomaterial-based technical platform to effectively enhance dopamine neural differentiation of ESCs, which can be potentially applied for cell transplantation therapy.
Volume 9(16)
Pages 2445-55
Published 2014-11-1
DOI 10.2217/nnm.13.197
PMID 24564300
MeSH Animals Cell Differentiation / drug effects* Dopaminergic Neurons / drug effects Embryonic Stem Cells / drug effects* Graphite / administration & dosage* Graphite / chemistry Mice Nanotubes, Carbon / chemistry* Oxides / administration & dosage Oxides / chemistry
Human and Animal Cells MC3T3-G2/PA6(RCB1127)