| RRC ID |
72609
|
| 著者 |
Murai R, Yamada K, Tanaka M, Kuribayashi K, Kobayashi D, Tsuji N, Watanabe N.
|
| タイトル |
Reconstructing a 3-dimensional image of the results of antinuclear antibody testing by indirect immunofluorescence.
|
| ジャーナル |
J Immunol Methods
|
| Abstract |
Indirect immunofluorescence anti-nuclear antibody testing (IIF-ANAT) is an essential screening tool in the diagnosis of various autoimmune disorders. ANA titer quantification and interpretation of immunofluorescence patterns are determined subjectively, which is problematic. First, we determined the examination conditions under which IIF-ANAT fluorescence intensities are quantified. Next, IIF-ANAT was performed using homogeneous, discrete speckled, and mixed serum samples. Images were obtained using Bio Zero BZ-8000, and 3-dimensional images were reconstructed using the BZ analyzer software. In the 2-dimensional analysis, homogeneous ANAs hid the discrete speckled pattern, resulting in a diagnosis of homogeneous immunofluorescence. However, 3-dimensional analysis of the same sample showed discrete speckled-type ANA in the homogeneous background. This study strengthened the current IIF-ANAT method by providing a new approach to quantify the fluorescence intensity and enhance the resolution of IIF-ANAT fluorescence patterns. Reconstructed 3-dimensional imaging of IIF-ANAT can be a powerful tool for routine laboratory examination.
|
| 巻・号 |
387(1-2)
|
| ページ |
312-6
|
| 公開日 |
2013-1-31
|
| DOI |
10.1016/j.jim.2012.10.004
|
| PII |
S0022-1759(12)00315-8
|
| PMID |
23073364
|
| MeSH |
Antibodies, Antinuclear / analysis
Antibodies, Antinuclear / blood
Antibodies, Antinuclear / immunology*
Autoimmune Diseases / blood
Autoimmune Diseases / diagnosis
Autoimmune Diseases / immunology*
Cell Line, Tumor
Fluorescent Antibody Technique, Indirect / methods*
Humans
Imaging, Three-Dimensional / methods*
Reproducibility of Results
Sensitivity and Specificity
Time Factors
|
| IF |
1.901
|
| リソース情報 |
| ヒト・動物細胞 |
HEp-2(RCB1889) |
