RRC ID 72626
Author Zhou Z, Katoh Y, Nakayama K.
Title CEP19-RABL2-IFT-B axis controls BBSome-mediated ciliary GPCR export.
Journal Mol Biol Cell
Abstract The intraflagellar transport (IFT) machinery mediates the import and export of ciliary proteins across the ciliary gate, as well as bidirectional protein trafficking within cilia. In addition to ciliary anterograde protein trafficking, the IFT-B complex participates in the export of membrane proteins together with the BBSome, which consists of eight subunits encoded by the causative genes of Bardet-Biedl syndrome (BBS). The IFT25-IFT27/BBS19 dimer in the IFT-B complex constitutes its interface with the BBSome. We here show that IFT25-IFT27 and the RABL2 GTPase bind the IFT74/BBS22-IFT81 dimer of the IFT-B complex in a mutually exclusive manner. Cells expressing GTP-locked RABL2 [RABL2(Q80L)], but not wild-type RABL2, phenocopied IFT27-knockout cells, i.e., they demonstrated BBS-associated ciliary defects, including accumulation of LZTFL1/BBS17 and the BBSome within cilia, and the suppression of export of the ciliary GPCRs GPR161 and Smoothened. RABL2(Q80L) enters cilia in a manner dependent on the basal body protein CEP19, but its entry into cilia is not necessary for causing BBS-associated ciliary defects. These observations suggest that GTP-bound RABL2 is likely to be required for recruitment of the IFT-B complex to the ciliary base, where it is replaced with IFT25-IFT27.
Volume 33(13)
Pages ar126
Published 2022-11-1
DOI 10.1091/mbc.E22-05-0161
PMID 36074075
PMC PMC9634966
MeSH Bardet-Biedl Syndrome* / genetics Bardet-Biedl Syndrome* / metabolism Cell Cycle Proteins / metabolism Cilia* / metabolism Cytoskeletal Proteins / metabolism Flagella / metabolism GTP Phosphohydrolases / metabolism Guanosine Triphosphate / metabolism Humans Membrane Proteins / metabolism Protein Transport / genetics
IF 3.791
Human and Animal Cells 293T(RCB2202)