RRC ID 72634
Author Zhang J, Miao D, Zhu WF, Xu J, Liu WY, Kitdamrongtham W, Manosroi J, Abe M, Akihisa T, Feng F.
Title Biological Activities of Phenolics from the Fruits of Phyllanthus emblica L. (Euphorbiaceae).
Journal Chem Biodivers
Abstract Seven phenolic compounds, 1 - 7, including a new organic acid gallate, mucic acid 1-ethyl 6-methyl ester 2-O-gallate (7), were isolated from the MeOH extract of the fruits of Phyllanthus emblica L. (Euphorbiaceae). The structures were elucidated on the basis of extensive spectroscopic analysis and comparison with literature data. Upon evaluated for their antioxidant abilities by 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. The inhibitory activities against melanogenesis in B16 melanoma cells induced by α-MSH, as well as cytotoxic activities against four human cancer cell lines were also evaluated. All phenolic compounds, 1 - 7, exhibited potent antioxidant abilities (DPPH: IC50 5.6 - 12.9 μm; ABTS: 0.87 - 8.43 μm Trolox/μm; FRAP: 1.01 - 5.79 μm Fe2+ /μm, respectively). Besides, 5 - 7, also exhibited moderate inhibitory activities against melanogenesis (80.7 - 86.8% melanin content), even with no or low toxicity to the cells (93.5 - 101.6% cell viability) at a high concentration of 100 μm. Compounds 1 - 3 exhibited cytotoxic activity against one or more cell lines (IC50 13.9 - 68.4%), and compound 1 with high tumor selectivity for A549 (SI 3.2).
Volume 14(12)
Published 2017-12-1
DOI 10.1002/cbdv.201700404
PMID 28960771
MeSH A549 Cells Animals Antineoplastic Agents, Phytogenic / chemistry* Antineoplastic Agents, Phytogenic / isolation & purification Antineoplastic Agents, Phytogenic / toxicity Antioxidants / chemistry Cell Line, Tumor Cell Survival / drug effects Fruit / chemistry Fruit / metabolism HL-60 Cells Humans Magnetic Resonance Spectroscopy Melanins / metabolism Mice Molecular Conformation Phenols / chemistry* Phenols / isolation & purification Phenols / pharmacology Phyllanthus emblica / chemistry* Phyllanthus emblica / metabolism Plant Extracts / chemistry
IF 2.039
Human and Animal Cells HL60 A549 WI-38