RRC ID 72656
Author Deliu LP, Turingan M, Jadir D, Lee B, Ghosh A, Grewal SS.
Title Serotonergic neuron ribosomal proteins regulate the neuroendocrine control of Drosophila development.
Journal PLoS Genet
Abstract The regulation of ribosome function is a conserved mechanism of growth control. While studies in single cell systems have defined how ribosomes contribute to cell growth, the mechanisms that link ribosome function to organismal growth are less clear. Here we explore this issue using Drosophila Minutes, a class of heterozygous mutants for ribosomal proteins. These animals exhibit a delay in larval development caused by decreased production of the steroid hormone ecdysone, the main regulator of larval maturation. We found that this developmental delay is not caused by decreases in either global ribosome numbers or translation rates. Instead, we show that they are due in part to loss of Rp function specifically in a subset of serotonin (5-HT) neurons that innervate the prothoracic gland to control ecdysone production. We find that these effects do not occur due to altered protein synthesis or proteostasis, but that Minute animals have reduced expression of synaptotagmin, a synaptic vesicle protein, and that the Minute developmental delay can be partially reversed by overexpression of synaptic vesicle proteins in 5-HTergic cells. These results identify a 5-HT cell-specific role for ribosomal function in the neuroendocrine control of animal growth and development.
Volume 18(9)
Pages e1010371
Published 2022-9-1
DOI 10.1371/journal.pgen.1010371
PMID 36048889
PMC PMC9473637
MeSH Animals Drosophila* / genetics Drosophila Proteins* / metabolism Drosophila melanogaster / metabolism Ecdysone / metabolism Gene Expression Regulation, Developmental Larva Ribosomal Proteins / genetics Ribosomal Proteins / metabolism Serotonergic Neurons / metabolism Serotonin / genetics Serotonin / metabolism
IF 5.175