RRC ID 72690
著者 Kawakami K, Nagao R, Tahara YO, Hamaguchi T, Suzuki T, Dohmae N, Kosumi D, Shen JR, Miyata M, Yonekura K, Kamiya N.
タイトル Structural implications for a phycobilisome complex from the thermophilic cyanobacterium Thermosynechococcus vulcanus.
ジャーナル Biochim Biophys Acta Bioenerg
Abstract Phycobilisomes (PBSs) are huge, water-soluble light-harvesting complexes used by oxygenic photosynthetic organisms. The structures of some subunits of the PBSs, including allophycocyanin (APC) and phycocyanin (PC), have been solved by X-ray crystallography previously. However, there are few reports on the overall structures of PBS complexes in photosynthetic organisms. Here, we report the overall structure of the PBS complex isolated from the cyanobacterium Thermosynechococcus vulcanus, determined by negative-staining electron microscopy (EM). Intact PBS complexes were purified by trehalose density gradient centrifugation with a high-concentration phosphate buffer and then subjected to a gradient-fixation preparation using glutaraldehyde. The final map constructed by the single-particle analysis of EM images showed a hemidiscoidal structure of the PBS, consisting of APC cores and peripheral PC rods. The APC cores are composed of five cylinders: A1, A2, B, C1, and C2. Each of the cylinders is composed of three (A1 and A2), four (B), or two (C1 and C2) APC trimers. In addition, there are eight PC rods in the PBS: one bottom pair (Rb and Rb'), one top pair (Rt and Rt'), and two side pairs (Rs1/Rs1' and Rs2/Rs2'). Comparison with the overall structures of PBSs from other organisms revealed structural characteristics of T. vulcanus PBS.
巻・号 1862(9)
ページ 148458
公開日 2021-9-1
DOI 10.1016/j.bbabio.2021.148458
PII S0005-2728(21)00091-8
PMID 34062150
MeSH Amino Acid Sequence Crystallography, X-Ray Mass Spectrometry Microscopy, Electron, Transmission Molecular Docking Simulation Phycobilisomes / chemistry* Phycocyanin / chemistry* Thermosynechococcus / chemistry
IF 4.441
リソース情報
藻類 NIES-2134