| RRC ID |
72804
|
| Author |
Kanadome T, Hayashi K, Seto Y, Eiraku M, Nakajima K, Nagai T, Matsuda T.
|
| Title |
Development of intensiometric indicators for visualizing N-cadherin interaction across cells.
|
| Journal |
Commun Biol
|
| Abstract |
N-cadherin (NCad) is a classical cadherin that mediates cell-cell interactions in a Ca2+-dependent manner. NCad participates in various biological processes, from ontogenesis to higher brain functions, though the visualization of NCad interactions in living cells remains limited. Here, we present intensiometric NCad interaction indicators, named INCIDERs, that utilize dimerization-dependent fluorescent proteins. INCIDERs successfully visualize reversible NCad interactions across cells. Compared to FRET-based indicators, INCIDERs have a ~70-fold higher signal contrast, enabling clear identification of NCad interactions. In primary neuronal cells, NCad interactions are visualized between closely apposed processes. Furthermore, visualization of NCad interaction at cell adhesion sites in dense cell populations is achieved by two-photon microscopy. INCIDERs are useful tools in the spatiotemporal investigation of NCad interactions across cells; future research should evaluate the potential of INCIDERs in mapping complex three-dimensional architectures in multi-cellular systems.
|
| Volume |
5(1)
|
| Pages |
1065
|
| Published |
2022-10-7
|
| DOI |
10.1038/s42003-022-04023-2
|
| PII |
10.1038/s42003-022-04023-2
|
| PMID |
36207396
|
| PMC |
PMC9546846
|
| MeSH |
Cadherins* / metabolism
Cell Adhesion
Neurons* / metabolism
|
| IF |
4.165
|
| Resource |
| Human and Animal Cells |
293T(RCB2202)
COS-7(RCB0539)
K562 |
