RRC ID 72805
著者 Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S.
タイトル Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model.
ジャーナル Sci Rep
Abstract Intravenous infusion of stem cells is a minimally invasive cellular delivery method, though a few have been reported in a critical limb-threatening ischemia (CLTI) animal model or patients. In the present study, we hypothesized that intravenous infusion of bone-marrow derived mesenchymal stem cells (MSCs) improves tissue perfusion in a rat hindlimb ischemia model. Hindlimb ischemia was generated in Sprague-Dawley rats by femoral artery removal, then seven days after ischemic induction intravenous infusion of 1 × 106 MSCs (cell group) or vehicle (control group) was performed. As compared with the control, tissue perfusion was significantly increased in the cell group. Histological findings showed that capillary density was significantly increased in the cell group, with infused green fluorescent protein (GFP)-MSCs distributed in the ischemic limb. Furthermore, gene expression of vascular endothelial growth factor (VEGF) was significantly increased in ischemic hindlimb muscle tissues of rats treated with MSC infusion. In conclusion, intravenous infusion of bone-marrow derived MSCs improved tissue perfusion in ischemic hindlimbs through angiogenesis, suggesting that intravenous infusion of MSCs was a promising cell delivery method for treatment of CLTI.
巻・号 12(1)
ページ 16986
公開日 2022-10-10
DOI 10.1038/s41598-022-18485-1
PII 10.1038/s41598-022-18485-1
PMID 36216855
PMC PMC9551049
MeSH Animals Bone Marrow / metabolism Disease Models, Animal Green Fluorescent Proteins / metabolism Hindlimb / blood supply Infusions, Intravenous Ischemia / pathology Lower Extremity Mesenchymal Stem Cell Transplantation* / methods Mesenchymal Stem Cells* / metabolism Neovascularization, Physiologic / physiology Perfusion Peripheral Vascular Diseases* Rats Rats, Sprague-Dawley Vascular Endothelial Growth Factor A / genetics Vascular Endothelial Growth Factor A / metabolism
IF 3.998
リソース情報
ラット W-Tg(CAG-GFP)184Ys (StrainID=525)