RRC ID |
72805
|
著者 |
Maeda S, Kawamura T, Sasaki M, Shimamura K, Shibuya T, Harada A, Honmou O, Sawa Y, Miyagawa S.
|
タイトル |
Intravenous infusion of bone marrow-derived mesenchymal stem cells improves tissue perfusion in a rat hindlimb ischemia model.
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ジャーナル |
Sci Rep
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Abstract |
Intravenous infusion of stem cells is a minimally invasive cellular delivery method, though a few have been reported in a critical limb-threatening ischemia (CLTI) animal model or patients. In the present study, we hypothesized that intravenous infusion of bone-marrow derived mesenchymal stem cells (MSCs) improves tissue perfusion in a rat hindlimb ischemia model. Hindlimb ischemia was generated in Sprague-Dawley rats by femoral artery removal, then seven days after ischemic induction intravenous infusion of 1 × 106 MSCs (cell group) or vehicle (control group) was performed. As compared with the control, tissue perfusion was significantly increased in the cell group. Histological findings showed that capillary density was significantly increased in the cell group, with infused green fluorescent protein (GFP)-MSCs distributed in the ischemic limb. Furthermore, gene expression of vascular endothelial growth factor (VEGF) was significantly increased in ischemic hindlimb muscle tissues of rats treated with MSC infusion. In conclusion, intravenous infusion of bone-marrow derived MSCs improved tissue perfusion in ischemic hindlimbs through angiogenesis, suggesting that intravenous infusion of MSCs was a promising cell delivery method for treatment of CLTI.
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巻・号 |
12(1)
|
ページ |
16986
|
公開日 |
2022-10-10
|
DOI |
10.1038/s41598-022-18485-1
|
PII |
10.1038/s41598-022-18485-1
|
PMID |
36216855
|
PMC |
PMC9551049
|
MeSH |
Animals
Bone Marrow / metabolism
Disease Models, Animal
Green Fluorescent Proteins / metabolism
Hindlimb / blood supply
Infusions, Intravenous
Ischemia / pathology
Lower Extremity
Mesenchymal Stem Cell Transplantation* / methods
Mesenchymal Stem Cells* / metabolism
Neovascularization, Physiologic / physiology
Perfusion
Peripheral Vascular Diseases*
Rats
Rats, Sprague-Dawley
Vascular Endothelial Growth Factor A / genetics
Vascular Endothelial Growth Factor A / metabolism
|
IF |
3.998
|
リソース情報 |
ラット |
W-Tg(CAG-GFP)184Ys (StrainID=525) |