| RRC ID |
72837
|
| Author |
Nagai K, Tahara-Hanaoka S, Morishima Y, Tokunaga T, Imoto Y, Noguchi E, Kanemaru K, Imai M, Shibayama S, Hizawa N, Fujieda S, Yamagata K, Shibuya A.
|
| Title |
Expression and function of Allergin-1 on human primary mast cells.
|
| Journal |
PLoS One
|
| Abstract |
Mast cells (MC) play an important role in allergic and non-allergic immune responses. Activation of human MC is modulated by several cell surface inhibitory receptors, including recently identified Allergin-1 expressed on both human and mouse MC. Although Allergin-1 suppresses IgE-mediated, mast cell-dependent anaphylaxis in mice, the expression profile and function of Allergin-1 on human primary MC remains undetermined. Here, we established a seven-color flow cytometry method for assessing expression and function of a very small number of human primary MC. We show that Allergin-1S1, a splicing isoform of Allergin-1, is predominantly expressed on human primary MC in both bronchoalveolar lavage (BAL) fluid and nasal scratching specimens. Moreover, Allergin-1S1 inhibits IgE-mediated activation from human primary MC in BAL fluid. These results indicate that Allergin-1 on human primary MC exhibits similar characteristics as mouse Allergin-1 in the expression profile and function.
|
| Volume |
8(10)
|
| Pages |
e76160
|
| Published |
2013-1-1
|
| DOI |
10.1371/journal.pone.0076160
|
| PII |
PONE-D-13-27350
|
| PMID |
24116093
|
| PMC |
PMC3792105
|
| MeSH |
Bronchoalveolar Lavage Fluid / cytology
Flow Cytometry
Humans
Immunoglobulin E / immunology
Immunoglobulin E / metabolism
Mast Cells / cytology
Mast Cells / immunology
Mast Cells / metabolism*
Receptors, Immunologic / genetics
Receptors, Immunologic / metabolism*
|
| IF |
2.74
|
| Resource |
| Cord blood stem cells for research |
|
