RRC ID 72844
Author Inoue S, Orimo A, Hosoi T, Kondo S, Toyoshima H, Kondo T, Ikegami A, Ouchi Y, Orimo H, Muramatsu M.
Title Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
Journal Proc Natl Acad Sci U S A
Abstract Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive gene that encodes a putative zinc finger protein. It has a RING finger motif present in a family of apparent DNA-binding proteins and is designated estrogen-responsive finger protein (efp). efp cDNA contains a consensus estrogen-responsive element at the 3' untranslated region that can act as a downstream estrogen-dependent enhancer. Moreover, efp is regulated by estrogen as demonstrated at both the mRNA and the protein level in ER-positive cells derived from mammary gland. These data suggest that efp may represent an estrogen-responsive transcription factor that mediates phenotypic expression of the diverse estrogen action. Thus, the genomic binding-site cloning may be applicable for isolation of the target genes of other transcription factors.
Volume 90(23)
Pages 11117-21
Published 1993-12-1
DOI 10.1073/pnas.90.23.11117
PMID 8248217
PMC PMC47933
MeSH Amino Acid Sequence Base Sequence Blotting, Northern Blotting, Western Cloning, Molecular Consensus Sequence DNA, Complementary / genetics DNA-Binding Proteins / chemistry DNA-Binding Proteins / genetics* Gene Expression Gene Expression Regulation Genes Molecular Sequence Data Molecular Weight RNA, Messenger / genetics Receptors, Estrogen / genetics* Regulatory Sequences, Nucleic Acid Sequence Alignment Sequence Homology, Amino Acid Transcription Factors / chemistry Transcription Factors / genetics* Zinc Fingers*
IF 9.412
Human and Animal Cells HBL-100(RCB0460)