| RRC ID |
72844
|
| 著者 |
Inoue S, Orimo A, Hosoi T, Kondo S, Toyoshima H, Kondo T, Ikegami A, Ouchi Y, Orimo H, Muramatsu M.
|
| タイトル |
Genomic binding-site cloning reveals an estrogen-responsive gene that encodes a RING finger protein.
|
| ジャーナル |
Proc Natl Acad Sci U S A
|
| Abstract |
Estrogen receptor (ER)-binding fragments were isolated from human genomic DNA by using a recombinant ER protein. Using one of these fragments as a probe, we have identified an estrogen-responsive gene that encodes a putative zinc finger protein. It has a RING finger motif present in a family of apparent DNA-binding proteins and is designated estrogen-responsive finger protein (efp). efp cDNA contains a consensus estrogen-responsive element at the 3' untranslated region that can act as a downstream estrogen-dependent enhancer. Moreover, efp is regulated by estrogen as demonstrated at both the mRNA and the protein level in ER-positive cells derived from mammary gland. These data suggest that efp may represent an estrogen-responsive transcription factor that mediates phenotypic expression of the diverse estrogen action. Thus, the genomic binding-site cloning may be applicable for isolation of the target genes of other transcription factors.
|
| 巻・号 |
90(23)
|
| ページ |
11117-21
|
| 公開日 |
1993-12-1
|
| DOI |
10.1073/pnas.90.23.11117
|
| PMID |
8248217
|
| PMC |
PMC47933
|
| MeSH |
Amino Acid Sequence
Base Sequence
Blotting, Northern
Blotting, Western
Cloning, Molecular
Consensus Sequence
DNA, Complementary / genetics
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
Gene Expression
Gene Expression Regulation
Genes
Molecular Sequence Data
Molecular Weight
RNA, Messenger / genetics
Receptors, Estrogen / genetics*
Regulatory Sequences, Nucleic Acid
Sequence Alignment
Sequence Homology, Amino Acid
Transcription Factors / chemistry
Transcription Factors / genetics*
Zinc Fingers*
|
| IF |
9.412
|
| リソース情報 |
| ヒト・動物細胞 |
HBL-100(RCB0460) |
