| RRC ID |
72861
|
| Author |
Ohno T, Wang X, Kurashima J, Saijo-Kurita K, Hirono M.
|
| Title |
A novel Vero cell line for use as a mammalian host-vector system in serum-free medium.
|
| Journal |
Cytotechnology
|
| Abstract |
We have established a novel cell line from a Vero cell derivative that is useful for expression of exogenous genes and protein production. Parental Vero-317 cells can grow in biotin-containing Eagle's MEM without supplements. By transforming this cell line with replication origin-defective SV40 DNA, which contains a temperature-sensitive tsA58 large T antigen gene, we established the Verots S3 cell line that amplified a SV40-origin containing plasmid. The cell line expressed a human growth hormone (hGH) gene insert with higher efficiency than COS-7 cells in 5% serum-containing MEM and could grow and continue hGH expression in protein-free MEM. However, temperature-sensitive shut down of hGH production was observed not immediately but 3 days after the temperature shift from 33 degrees C to 39.5 degrees C.
|
| Volume |
7(3)
|
| Pages |
165-72
|
| Published |
1991-11-1
|
| DOI |
10.1007/BF00365927
|
| PMID |
1368119
|
| MeSH |
Animals
Antigens, Polyomavirus Transforming / biosynthesis
Blotting, Southern
Cell Division
Cell Line
Culture Media, Serum-Free
Gene Expression
Genetic Vectors*
Growth Hormone / genetics
Growth Hormone / metabolism
Kinetics
Plasmids
Restriction Mapping
Temperature
Transfection
Transformation, Genetic
Vero Cells / cytology
Vero Cells / metabolism*
|
| IF |
1.777
|
| Resource |
| Human and Animal Cells |
Vero(RCB0001) |
