RRC ID 72871
Author Mang D, Mayu K, Toyama T, Yamagishi T, Sato R.
Title BmGr4 responds to sucrose and glucose and expresses in tachykinin-related peptide-secreting enteroendocrine cells.
Journal Insect Biochem Mol Biol
Abstract The regulatory hormones known as tachykinin-related peptides (TRPs) are identified as brain-gut peptides in insects. Dietary components from mulberry leaves, including glucose, induce secretion of TRPs from Bombyx mori midgut. However, the sensory molecules that recognize these compounds are still unknown. Here, we identified the gustatory receptor, BmGr4, as a sucrose and glucose receptor using Ca2+ imaging. Immunostaining revealed BmGr4 expression not only in the midgut, but also in the brain. In addition, BmGr4 expression was found to co-localize with TRP-expressing cells in both midgut enteroendocrine cells (EECs) and brain neurosecretory cells (NSCs). Furthermore, dietary nutrients after food intake result in an increase of TRP-level in hemolymph of silkworm larvae. These results provide significant circumstantial evidence for the involvement of the sucrose and glucose receptor, BmGr4, in the elicitation of TRP secretion in midgut EECs and brain NSCs.
Volume 150
Pages 103858
Published 2022-11-1
DOI 10.1016/j.ibmb.2022.103858
PII S0965-1748(22)00140-0
PMID 36244651
MeSH Animals Bombyx* / metabolism Enteroendocrine Cells / metabolism Glucose* / metabolism Larva / metabolism Sucrose / metabolism Sucrose / pharmacology Tachykinins / metabolism
IF 3.827
Resource
Human and Animal Cells 293T(RCB2202)