RRC ID 72881
Author Oka M, Ogita K, Ando H, Horikawa T, Hayashibe K, Saito N, Kikkawa U, Ichihashi M.
Title Deletion of specific protein kinase C subspecies in human melanoma cells.
Journal J Cell Physiol
Abstract It has been shown that tumor-promoting phorbol ester, 12-O-tetradecanoylphorbol-13-acetate (TPA), stimulates the proliferation of normal human melanocytes, whereas it inhibits the growth of human melanoma cell lines. The expression of protein kinase C (PKC) subspecies, the major intracellular receptors for TPA, was examined in normal melanocytes and the four melanoma cell lines HM3KO, MeWo, HMV-1, and G361. PKC was partially purified and then separated into subspecies by column chromatography on Mono Q and hydroxyapatite successively, and finally subjected to immunoblot analysis using antibodies specific for the PKC subspecies. Of the PKC subspecies examined, delta-, epsilon-, and zeta-PKC were detected in both normal melanocytes and the four melanoma cell lines. In contrast, both alpha-PKC and beta-PKC were expressed in normal melanocytes, whereas either alpha-PKC or beta-PKC was detected in melanoma cells. Specifically, HM3KO, MeWo, and HMV-1 cells were shown to contain alpha-PKC but not beta-PKC, while G361 cells expressed beta-PKC but not alpha-PKC. The growth of these melanoma cells was suppressed by TPA treatment, and the growth of the G361 cells lacking alpha-PKC was inhibited more efficiently than the other melanoma cell lines which lacked beta-PKC. It was further shown that beta-PKC was not detected in freshly isolated human primary or metastatic melanoma tissues. These results suggest that the expression of alpha-PKC or beta-PKC may be altered during the malignant transformation of normal melanocytes and that loss of alpha-PKC or beta-PKC may be related to the inhibitory effect of TPA on the growth of melanoma cells.
Volume 167(3)
Pages 406-12
Published 1996-6-1
DOI 10.1002/(SICI)1097-4652(199606)167:3<406::AID-JCP4>3.0.CO;2-T
PII 10.1002/(SICI)1097-4652(199606)167:3<406::AID-JCP4>3.0.CO;2-T
PMID 8655594
MeSH Cell Division / drug effects Chromatography Durapatite Humans Immunoblotting Isoenzymes / isolation & purification Isoenzymes / metabolism* Melanocytes / drug effects Melanocytes / enzymology Melanoma / enzymology* Melanoma / pathology Protein Kinase C / isolation & purification Protein Kinase C / metabolism* Tetradecanoylphorbol Acetate / pharmacology Tumor Cells, Cultured
IF 5.546
Resource
Human and Animal Cells HMV-I(RCB0004)