| RRC ID |
72884
|
| 著者 |
Osada H, Kono T, Miwa K, Yamada C.
|
| タイトル |
Phorbol-ester-stimulated human lymphoid cell lines produce a plasminogen activator modulator inducing cell-bound urokinase-type plasminogen activator in malignant tumor cell lines.
|
| ジャーナル |
Int J Cancer
|
| Abstract |
The importance of cell-associated plasminogen activation in tumor invasion and metastasis is becoming increasingly evident. To clarify the modulators of cell-associated plasminogen activation in malignant states, we have recently established an assay system utilizing endogenous plasminogen activators on the cell surface. In the present study using the assay system, we found that the conditioned medium from phorbol 12-myristate 13-acetate (PMA)-stimulated human lymphoid cell lines, HUT 78 and Raji, strongly enhanced plasminogen activator (PA) activity on the surface of human malignant tumor cell lines (WI-38 VAI3 2RA, A431, A549 and HT-1080). The enhancing effect was inhibited by the addition of actinomycin D. By gel filtration, the active substances in PMA-stimulated HUT 78- and Raji-conditioned media were eluted in similar fractions corresponding to molecular weights of 60 to 80 kDa. The active substance was heat-labile. The enhanced PA activities were completely inhibited by anti-urokinase-type plasminogen activator (uPA) IgG. Moreover, the active substance was found to increase in cell-bound uPA antigen. These findings suggest that a population of activated lymphocytes produces a plasminogen activator modulator that induces uPA on the surface of malignant tumor cells.
|
| 巻・号 |
65(2)
|
| ページ |
178-85
|
| 公開日 |
1996-1-17
|
| DOI |
10.1002/(SICI)1097-0215(19960117)65:2<178::AID-IJC9>3.0.CO;2-W
|
| PII |
10.1002/(SICI)1097-0215(19960117)65:2<178::AID-IJC9>3.0.CO;2-W
|
| PMID |
8567114
|
| MeSH |
Cell Line
Culture Media, Conditioned
Dactinomycin / pharmacology
Humans
Lymphocytes / drug effects
Lymphocytes / metabolism*
Tetradecanoylphorbol Acetate / pharmacology*
Tumor Cells, Cultured
Up-Regulation / drug effects*
Urokinase-Type Plasminogen Activator / biosynthesis*
|
| IF |
5.145
|
| リソース情報 |
| ヒト・動物細胞 |
A431(RCB0202)
A549(RCB0098) |
