RRC ID 72887
著者 Sumi-Ichinose C, Hasegawa S, Ohtsuki M, Nomura H, Nomura T, Hagino Y, Fujita K, Nagatsu T.
タイトル Analysis of an alternative promoter that regulates tissue-specific expression of the human aromatic L-amino acid decarboxylase gene in cultured cell lines.
ジャーナル J Neural Transm (Vienna)
Abstract The human aromatic L-amino acid decarboxylase (AADC) gene is transcribed in a tissue-specific manner by an alternative promoter. In this study using human cultured cell lines, we analyzed the alternative promoter that regulates tissue-specific expression of AADC. Neither neuronal nor nonneuronal-type mRNA of AADC was detected in HeLa cells, nonneuronal-type mRNA of AADC was expressed in HepG2 cells, and the neuronal-type was expressed in the SK-N-SH cell line. We examined the promoter activities located in 5'- and 3'-flanking regions of exon N1 and exon L1 by transfection experiments. Plasmids containing 5'-flanking regions of exon L1, the shortest of which was 0.3 kb, could promote specifically high expression of the reporter gene HepG2 cells. On the other hand, plasmids containing 5'-flanking regions of exon N1 (3.6 kb to 0.5 kb) could promote the reporter gene expression not only in SK-N-SH cells but also in HeLa and HepG2. More enhanced expression were observed by transfection of plasmids containing parts of the first intron in these cell lines. Thus, these results suggest that the basal liver-specific promoter activity is located in the 5'-flanking region of exon L1 and that the first intron may also be needed for enhanced expression rather than determination of cell-specificity.
巻・号 103(1-2)
ページ 1-15
公開日 1996-1-1
DOI 10.1007/BF01292612
PMID 9026364
MeSH Aromatic-L-Amino-Acid Decarboxylases / biosynthesis* Aromatic-L-Amino-Acid Decarboxylases / genetics* Cell Line Chromatin / ultrastructure Deoxyribonuclease I / metabolism Exons Gene Expression Regulation, Enzymologic / physiology* HeLa Cells Humans Plasmids Polymerase Chain Reaction Promoter Regions, Genetic* RNA, Messenger / biosynthesis Transfection
IF 3.505
リソース情報
ヒト・動物細胞 SK-N-SH(RCB0426) Hep G2(RCB0459) HeLa(RCB0007)