RRC ID 72910
Author Ohye T, Hori TA, Katoh S, Nagatsu T, Ichinose H.
Title Genomic organization and chromosomal localization of the human sepiapterin reductase gene.
Journal Biochem Biophys Res Commun
Abstract Sepiapterin reductase (SPR) catalyzes the final step of the biosynthetic pathway of tetrahydrobiopterin, which is an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases. To aid the analysis of any possible human diseases caused by mutations in SPR, we have cloned and characterized the human SPR gene. The gene is composed of three exons spanning approximately 4 kilobases. The transcriptional starting point was determined around the cytosine nucleotide at position -81 by primer extension and RT-PCR analyses. There was no typical TATA-box within 300 bp from the transcriptional starting point. We found the Sp1-binding consensus sequence in the 5'-flanking region. The human SPR gene was mapped to chromosome band 2p13 by fluorescence in situ hybridization.
Volume 251(2)
Pages 597-602
Published 1998-10-20
DOI 10.1006/bbrc.1998.9503
PII S0006-291X(98)99503-1
PMID 9792819
MeSH Alcohol Oxidoreductases / genetics* Amino Acid Sequence Base Sequence Binding Sites Chromosome Mapping Chromosomes, Human, Pair 2* Consensus Sequence DNA Primers Exons Genomic Library Humans In Situ Hybridization, Fluorescence Molecular Sequence Data Restriction Mapping Reverse Transcriptase Polymerase Chain Reaction Sp1 Transcription Factor / metabolism TATA Box Transcription, Genetic Tumor Cells, Cultured
IF 2.985
Human and Animal Cells Hep G2(RCB0459)