論文 - 詳細
| RRC ID | 72910 |
|---|---|
| 著者 | Ohye T, Hori TA, Katoh S, Nagatsu T, Ichinose H. |
| タイトル | Genomic organization and chromosomal localization of the human sepiapterin reductase gene. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
Sepiapterin reductase (SPR) catalyzes the final step of the biosynthetic pathway of tetrahydrobiopterin, which is an essential cofactor for aromatic amino acid hydroxylases and nitric oxide synthases. To aid the analysis of any possible human diseases caused by mutations in SPR, we have cloned and characterized the human SPR gene. The gene is composed of three exons spanning approximately 4 kilobases. The transcriptional starting point was determined around the cytosine nucleotide at position -81 by primer extension and RT-PCR analyses. There was no typical TATA-box within 300 bp from the transcriptional starting point. We found the Sp1-binding consensus sequence in the 5'-flanking region. The human SPR gene was mapped to chromosome band 2p13 by fluorescence in situ hybridization. |
| 巻・号 | 251(2) |
| ページ | 597-602 |
| 公開日 | 1998-10-20 |
| DOI | 10.1006/bbrc.1998.9503 |
| PII | S0006-291X(98)99503-1 |
| PMID | 9792819 |
| MeSH | Alcohol Oxidoreductases / genetics* Amino Acid Sequence Base Sequence Binding Sites Chromosome Mapping Chromosomes, Human, Pair 2* Consensus Sequence DNA Primers Exons Genomic Library Humans In Situ Hybridization, Fluorescence Molecular Sequence Data Restriction Mapping Reverse Transcriptase Polymerase Chain Reaction Sp1 Transcription Factor / metabolism TATA Box Transcription, Genetic Tumor Cells, Cultured |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Wikipedia |
| 各媒体での言及数の合計 | 3 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) |