RRC ID 72918
Author Sasaki E, Pai R, Halter F, Komurasaki T, Arakawa T, Kobayashi K, Kuroki T, Tarnawski AS.
Title Induction of cyclooxygenase-2 in a rat gastric epithelial cell line by epiregulin and basic fibroblast growth factor.
Journal J Clin Gastroenterol
Abstract Prostaglandins play an important role in maintaining gastric mucosal integrity. Cyclooxygenases (COX-1 and -2) are the key enzymes involved in prostaglandin synthesis. COX-2 expression in gastric epithelial cells remains a subject of controversy, and a possible regulation of gastric COX-2 by growth factors has not been explored. Therefore, we studied the effect of growth factors including epiregulin, basic fibroblast growth factor (bFGF), and vascular endothelial growth factor (VEGF) on expression of COX-2 in a gastric epithelial cell line (RGM1) derived from normal rat gastric mucosa. Cells were incubated with 10 or 100 ng/ml of EGF. epiregulin, bFGF, or VEGF for 1, 2, 3, 6, and 24 h. COX-2 mRNA expression was determined by RT-PCR using specific COX-2 primers and COX-2 protein expression was determined by Western blotting. This study showed that COX-2 mRNA and protein are expressed in the gastric epithelial RGM1 cell line and that epiregulin and bFGF (but not VEGF) significantly increase expression of COX-2 mRNA and protein. Because PGs play an important role in mucosal defense, this study suggests that some growth factors contribute to maintaining mucosal integrity via activation of the COX-2 gene.
Volume 27 Suppl 1
Pages S21-7
Published 1998-1-1
DOI 10.1097/00004836-199800001-00005
PMID 9872494
MeSH Animals Blotting, Western Cell Line Cyclooxygenase 2 DNA Primers Endothelial Growth Factors / metabolism* Epidermal Growth Factor / metabolism* Epiregulin Fibroblast Growth Factor 2 / metabolism* Gastric Mucosa / cytology Gastric Mucosa / enzymology* Gene Expression Regulation, Enzymologic Isoenzymes / genetics Isoenzymes / metabolism* Lymphokines / metabolism* Prostaglandin-Endoperoxide Synthases / genetics Prostaglandin-Endoperoxide Synthases / metabolism* RNA, Messenger / biosynthesis Rats Rats, Sprague-Dawley Reverse Transcriptase Polymerase Chain Reaction Vascular Endothelial Growth Factor A Vascular Endothelial Growth Factors
IF 2.973
Human and Animal Cells RGM1(RCB0876)