論文 - 詳細
| RRC ID | 72920 |
|---|---|
| 著者 | Tsurushima H, Ohno T, Tsuboi K, Yoshii Y, Meguro K, Nose T. |
| タイトル | [Low incidence of point mutation of N-ras oncogene in human gliomas]. |
| ジャーナル | No Shinkei Geka |
| Abstract |
We examined the incidence of point mutations in codon 12 and 61 of N-ras gene in human gliomas using PCR with mismatched primers. This method detects point mutations. PCR with mismatched primers induced restriction sites in normal DNA but not in mutational DNA. Genomic DNAs were extracted from paraffin-embedded tissues and were amplified with nested PCR. Among 17 cases, point mutation has not been able to be found so far, when examined in codon 12 of N-ras gene and among 10 cases in codon 61 of N-ras gene. It can thus be said that point mutational activation of N-ras oncogene is an uncommon event in human gliomas. |
| 巻・号 | 23(7) |
| ページ | 581-6 |
| 公開日 | 1995-7-1 |
| PMID | 7637839 |
| MeSH | Base Sequence Brain Neoplasms / genetics* Codon DNA, Neoplasm / genetics Genes, ras / genetics* Glioblastoma / genetics Glioma / genetics* Humans Molecular Sequence Data Point Mutation* Polymerase Chain Reaction |
| リソース情報 | |
| ヒト・動物細胞 | HL60(RCB0041) MOLT-4(RCB0206) |