| RRC ID |
72937
|
| 著者 |
Yazawa T, Kamma H, Fujiwara M, Matsui M, Horiguchi H, Satoh H, Fujimoto M, Yokoyama K, Ogata T.
|
| タイトル |
Lack of class II transactivator causes severe deficiency of HLA-DR expression in small cell lung cancer.
|
| ジャーナル |
J Pathol
|
| Abstract |
Small cell lung cancer (SCLC) is characteristically not associated with tumour-infiltrating lymphocytes. Since SCLC has been reported to show marked reduction of class I HLA, the reduced expression has been considered a means of escaping anti-cancer immunity. However, HLA-DR expressed in cancer cells is now known to contribute to anti-cancer immunity. To clarify the difference in HLA-DR expression between SCLC and non-small cell lung cancer (NSCLC), and the mechanism, the expression and the cis- and trans-acting factors involved were investigated. HLA-DR was not immunohistochemically detected in any SCLC and could not be induced by interferon gamma (IFN-gamma) in any SCLC cell line, whereas HLA-DR was expressed to varying degrees and was easily induced in NSCLC. SCLC cell lines lacked class II transactivator (CIITA) even after IFN-gamma induction, whereas NSCLC cell lines expressed CIITA. The other class II HLA-specific transcription factors were expressed and genomic DNA of HLA-DR, including the promoter, was conserved well both in SCLC and in NSCLC cell lines. CIITA transfection improved the expression of HLA-DR in SCLC. In conclusion, the lack of CIITA results in severe deficiency of HLA-DR expression in SCLC. Since CIITA has also been reported to induce class I HLA, CIITA transfection might make it possible to establish effective anti-cancer immunotherapy against SCLC through the up-regulation of class I and class II HLA.
|
| 巻・号 |
187(2)
|
| ページ |
191-9
|
| 公開日 |
1999-1-1
|
| DOI |
10.1002/(SICI)1096-9896(199901)187:2<191::AID-PATH206>3.0.CO;2-3
|
| PII |
10.1002/(SICI)1096-9896(199901)187:2<191::AID-PATH206>3.0.CO;2-3
|
| PMID |
10365094
|
| MeSH |
Blotting, Northern
Blotting, Southern
Carcinoma, Non-Small-Cell Lung / immunology
Carcinoma, Small Cell / genetics
Carcinoma, Small Cell / immunology*
Gene Expression
HLA-DR Antigens / genetics
HLA-DR Antigens / metabolism*
Humans
Interferon-gamma / immunology
Lung Neoplasms / genetics
Lung Neoplasms / immunology*
Nuclear Proteins*
RNA, Messenger / genetics
Recombinant Proteins
Trans-Activators / deficiency*
Trans-Activators / genetics
Transfection
Tumor Cells, Cultured
|
| IF |
6.021
|
| リソース情報 |
| ヒト・動物細胞 |
SCLC cell lines (Lu cells) |
