論文 - 詳細
| RRC ID | 72944 |
|---|---|
| 著者 | Tanaka T, Takimoto T, Morishige J, Kikuta Y, Sugiura T, Satouchi K. |
| タイトル | Non-methylene-interrupted polyunsaturated fatty acids: effective substitute for arachidonate of phosphatidylinositol. |
| ジャーナル | Biochem Biophys Res Commun |
| Abstract |
In mammalian tissues and cells, a characteristic of phosphatidylinositol (PI) is a high abundance of arachidonic acid (AA) relative to the other phospholipids. In this study, we investigated the effects of supplementation of several polyunsaturated fatty acids (PUFAs) on the AA concentration of the PI fraction using a cultured cell system. Neither alpha-linolenic acid nor eicosapentaenoic acid supplement reduced the level of AA in PI of HepG2 cells. In contrast to the n-3 series PUFAs, adding podocarpic acid (20:3, Delta-5,11,14) and pinolenic acid (18:3, Delta-5,9,12) reduced the AA content of the PI fraction from a control value of 15.9% to 7.0 and 8.7%, respectively. In the experiments with pinolenic acid, selective and significant accumulation of 20:3 (Delta-7,11,14), the chain-elongated metabolite of pinolenic acid, was observed in the PI fraction. On the other hand, adding columbinic acid (18:3, Delta-5t,9,12) had no effect on AA content of the PI fraction. Because both podocarpic acid and pinolenic acid are non-methylene-interrupted fatty acids (NMIFAs) that are not converted to AA metabolically, these NMIFAs may be interesting experimental tools for research on the function of PI-origin bioactive lipids. |
| 巻・号 | 264(3) |
| ページ | 683-8 |
| 公開日 | 1999-11-2 |
| DOI | 10.1006/bbrc.1999.1559 |
| PII | S0006291X99915590 |
| PMID | 10543992 |
| MeSH | Arachidonic Acid / chemistry* Arachidonic Acid / metabolism Fatty Acids, Unsaturated / chemistry* Fatty Acids, Unsaturated / metabolism Humans Mass Spectrometry Phosphatidylinositols / chemistry* Phosphatidylinositols / metabolism Tumor Cells, Cultured |
| IF | 2.985 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | Patent(IFI CLAIMS) |
| 各媒体での言及数の合計 | 4 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | Hep G2(RCB0459) |