論文 - 詳細
| RRC ID | 72950 |
|---|---|
| 著者 | Kaneko T, Matsui H, Shimokawa O, Nakahara A, Hyodo I. |
| タイトル | Cellular membrane fluidity measurement by fluorescence polarization in indomethacin-induced gastric cellular injury in vitro. |
| ジャーナル | J Gastroenterol |
| Abstract |
BACKGROUND:Gastric complications of indomethacin involve generation of reactive oxygen species, which induce gastric mucosal injury via lipid peroxidation of cell membranes. Peroxidation by reactive oxygen species alters the amounts of unsaturated fatty acids in the cell membrane and thus affects membrane fluidity. Indomethacin-induced lipid peroxidation can thus be detected by measuring cellular membrane fluidity by the fluorescence polarization (FP) method. The aim of this study was to elucidate the usefulness of the FP method for detecting indomethacin-induced gastric cellular injury in RGM-1 cells. METHODS:Indomethacin-treated RGM-1 cells were investigated by conventional cytotoxicity assay, fluorometry of diphenyl-1-pyrenylphosphine (DPPP) to detect lipid peroxidation, and FP. The effects of both a radical scavenger and an initiator on membrane fluidity change (MFC) in RGM-1 cells were examined. The sensitivity of FP in detecting cellular injury was compared with those of DPPP fluorometry and conventional cytotoxicity measurements. RESULTS:Indomethacin caused an increase in MFC as determined by FP before cytotoxicity was detected by conventional methods. The increase in MFC was associated with increased membrane phospholipid peroxidation (MPP) but not with a prostaglandin deficiency, and the increases in both MFC and MPP were prevented by vitamin E. CONCLUSIONS:The FP method is potentially useful for detecting cellular injury in vitro. |
| 巻・号 | 42(12) |
| ページ | 939-46 |
| 公開日 | 2007-12-1 |
| DOI | 10.1007/s00535-007-2116-y |
| PMID | 18085350 |
| MeSH | Animals Cell Line Epithelial Cells / chemistry Epithelial Cells / drug effects Fluorescence Polarization / methods* Gastric Mucosa / drug effects* Indomethacin / toxicity* Membrane Fluidity / drug effects* Membrane Lipids / metabolism Organophosphorus Compounds / analysis Phospholipids / metabolism Pyrenes / analysis Rats Sensitivity and Specificity Vitamin E / pharmacology |
| IF | 6.132 |
| リソース情報 | |
| ヒト・動物細胞 | RGM1(RCB0876) |