| RRC ID |
72953
|
| 著者 |
Shimokawa O, Matsui H, Nagano Y, Kaneko T, Shibahara T, Nakahara A, Hyodo I, Yanaka A, Majima HJ, Nakamura Y, Matsuzaki Y.
|
| タイトル |
Neoplastic transformation and induction of H+,K+ -adenosine triphosphatase by N-methyl-N'-nitro-N-nitrosoguanidine in the gastric epithelial RGM-1 cell line.
|
| ジャーナル |
In Vitro Cell Dev Biol Anim
|
| Abstract |
N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) induces gastric cancer in animal models. We established an MNNG-induced mutant of the rat murine RGM-1 gastric epithelial cell line, which we named RGK-1, that could be used as an in vitro model of gastric cancer. This cell line showed signs of neoplasia and transformation, in that it lost contact inhibition and formed tumors in nude mice. The mutant cells also expressed parietal cell-specific H(+),K(+)-adenosine triphosphatase (H(+),K(+)-ATPase), which parent RGM-1 did not. The results suggested that parent RGM-1 cells were gastric progenitor cells. This mutant RGK-1 cell line will contribute to future investigation on gastric carcinogenesis and to the development of other pathophysiologic fields.
|
| 巻・号 |
44(1-2)
|
| ページ |
26-30
|
| 公開日 |
2008-1-1
|
| DOI |
10.1007/s11626-007-9067-8
|
| PMID |
18034283
|
| MeSH |
Animals
Carcinogens / metabolism
Cell Line
Cell Transformation, Neoplastic*
Epithelial Cells / physiology*
Epithelial Cells / ultrastructure
Gastric Mucosa / cytology*
H(+)-K(+)-Exchanging ATPase / metabolism*
Methylnitronitrosoguanidine / metabolism*
Rats
Rats, Wistar
|
| IF |
1.665
|
| リソース情報 |
| ヒト・動物細胞 |
MKN45(RCB1001) |
