| RRC ID |
72975
|
| Author |
Park S, Lee S, Kim HS, Choi HJ, Jeong OC, Lin R, Cho Y, Lee MH.
|
| Title |
Square microchannel enables to focus and orient ellipsoidal Euglena gracilis cells by two-dimensional acoustic standing wave.
|
| Journal |
Mikrochim Acta
|
| Abstract |
Flow cytometry has become an indispensable tool for counting, analyzing, and sorting large cell populations in biological research and medical practice. Unfortunately, it has limitations in the analysis of non-spherically shaped cells due to the variation of their alignment with respect to the flow direction and, hence, the optical interrogation axis, resulting in unreliable cell analysis. Here, we present a simple on-chip acoustofluidic method to fix the orientation of ellipsoidal cells and focus them into a single, aligned stream. Specifically, by generating acoustic standing waves inside a 100 ⋅ 100 µm square-shaped microchannel, we successfully aligned and focused up to 97.7% of a population of Euglena gracilis (an ellipsoidal shaped microalgal species) cells in the center of the microchannel with high precision at a volume rate of 25 to 200 µL min-1. Uniform positioning of ellipsoidal cells is essential for making flow cytometry applicable to the investigation of a greater variety of cell populations and is expected to be beneficial for ecological studies and aquaculture.
|
| Volume |
189(9)
|
| Pages |
331
|
| Published |
2022-8-15
|
| DOI |
10.1007/s00604-022-05439-7
|
| PII |
10.1007/s00604-022-05439-7
|
| PMID |
35969307
|
| MeSH |
Acoustics
Euglena gracilis*
Flow Cytometry / methods
|
| Resource |
| Algae |
NIES-48 |
