Reference - Detail
RRC ID | 73072 |
---|---|
Author | Shoji M, Kuzuhara T. |
Title | Imaging Analysis of Neurotrophic Effects by CCN2 Protein in Neuronal Precursor Cells Derived from Human-Induced Pluripotent Stem Cells. |
Journal | Methods Mol Biol |
Abstract |
Human-induced pluripotent stem cells (hiPSCs) are useful tools to examine human neuronal maturation processes. In this chapter, we describe the maturation of human neuronal precursor cells derived from hiPSCs by cellular communication network family member 2, also known as connective tissue growth factor. We describe the (1) preparation of feeder cells for undifferentiated culture of hiPSCs, (2) undifferentiated culture of hiPSCs, (3) induction of neuronal precursor cells from hiPSCs, (4) maturation of neuronal precursor cells from hiPSCs, (5) immunofluorescent staining of neuronal cells from hiPSCs, and (6) immunofluorescence analysis. |
Volume | 2582 |
Pages | 269-280 |
Published | 2023-1-1 |
DOI | 10.1007/978-1-0716-2744-0_18 |
PMID | 36370356 |
MeSH | Cell Communication Cell Differentiation Connective Tissue Growth Factor / genetics Connective Tissue Growth Factor / metabolism Connective Tissue Growth Factor / pharmacology Feeder Cells Humans Induced Pluripotent Stem Cells* Neurons |
Resource | |
Human and Animal Cells | 201B7(HPS0063) |