RRC ID 73078
Author Awale S, Tezuka Y, Banskota AH, Adnyana IK, Kadota S.
Title Highly-oxygenated isopimarane-type diterpenes from Orthosiphon stamineus of Indonesia and their nitric oxide inhibitory activity.
Journal Chem Pharm Bull (Tokyo)
Abstract From the methanolic extract of Indonesian Orthosiphon stamineus, nine new highly-oxygenated isopimarane-type diterpenes [7-O-deacetylorthosiphol B (1), 6-hydroxyorthosiphol B (2), 3-O-deacetylorthosiphol I (3), 2-O-deacetylorthosiphol J (4), siphonols A-E (5-9)] have been isolated together with nine known diterpenes [orthosiphols H (10), K (11), M (12) and N (13); staminols A (14) and B (15); neoorthosiphols A (16) and B (17); norstaminol A (18)]. Their structures were determined based on the spectroscopic data. The isolated diterpenes inhibited nitric oxide (NO) production in lipopolysaccharide (LPS)-activated macrophage-like J774.1 cells. Compounds 4-7, 9, 10, 14, and 17 showed inhibitory activities more potent (IC(50), 10.8-25.5 microM) than a positive control N(G)-monomethyl-L-arginine (L-NMMA; IC(50), 26.0 microM).
Volume 51(3)
Pages 268-75
Published 2003-3-1
DOI 10.1248/cpb.51.268
PMID 12612410
MeSH Animals Carboxylic Acids / chemistry Carboxylic Acids / isolation & purification Carboxylic Acids / pharmacology* Cell Line Diterpenes / chemistry Diterpenes / isolation & purification Diterpenes / pharmacology* Indonesia Macrophages / cytology Macrophages / drug effects Macrophages / metabolism Mice Nitric Oxide / antagonists & inhibitors* Nitric Oxide / metabolism Orthosiphon* Oxygen / metabolism* Phenanthrenes / chemistry Phenanthrenes / isolation & purification Phenanthrenes / pharmacology* Plant Components, Aerial Plant Extracts / chemistry Plant Extracts / isolation & purification Plant Extracts / pharmacology
Resource
Human and Animal Cells J774.1(RCB0434)