| RRC ID |
73104
|
| Author |
Matsumura T, Totani H, Gunji Y, Fukuda M, Yokomori R, Deng J, Rethnam M, Yang C, Tan TK, Karasawa T, Kario K, Takahashi M, Osato M, Sanda T, Suda T.
|
| Title |
A Myb enhancer-guided analysis of basophil and mast cell differentiation.
|
| Journal |
Nat Commun
|
| Abstract |
The transcription factor MYB is a crucial regulator of hematopoietic stem and progenitor cells. However, the nature of lineage-specific enhancer usage of the Myb gene is largely unknown. We identify the Myb -68 enhancer, a regulatory element which marks basophils and mast cells. Using the Myb -68 enhancer activity, we show a population of granulocyte-macrophage progenitors with higher potential to differentiate into basophils and mast cells. Single cell RNA-seq demonstrates the differentiation trajectory is continuous from progenitors to mature basophils in vivo, characterizes bone marrow cells with a gene signature of mast cells, and identifies LILRB4 as a surface marker of basophil maturation. Together, our study leads to a better understanding of how MYB expression is regulated in a lineage-associated manner, and also shows how a combination of lineage-related reporter mice and single-cell transcriptomics can overcome the rarity of target cells and enhance our understanding of gene expression programs that control cell differentiation in vivo.
|
| Volume |
13(1)
|
| Pages |
7064
|
| Published |
2022-11-18
|
| DOI |
10.1038/s41467-022-34906-1
|
| PII |
10.1038/s41467-022-34906-1
|
| PMID |
36400777
|
| PMC |
PMC9674656
|
| MeSH |
Animals
Basophils*
Cell Differentiation / genetics
Hematopoiesis*
Leukocyte Count
Mice
Stem Cells / metabolism
|
| IF |
12.121
|
| Resource |
| Human and Animal Cells |
P-815(RCB1167) |
