Reference - Detail
| RRC ID | 73106 |
|---|---|
| Author | Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B. |
| Title | A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion. |
| Journal | J Cell Biol |
| Abstract |
Mammalian sperm-egg adhesion depends on the trans-interaction between the sperm-specific type I glycoprotein IZUMO1 and its oocyte-specific GPI-anchored receptor JUNO. However, the mechanisms and proteins (fusogens) that mediate the following step of gamete fusion remain unknown. Using live imaging and content mixing assays in a heterologous system and structure-guided mutagenesis, we unveil an unexpected function for IZUMO1 in cell-to-cell fusion. We show that IZUMO1 alone is sufficient to induce fusion, and that this ability is retained in a mutant unable to bind JUNO. On the other hand, a triple mutation in exposed aromatic residues prevents this fusogenic activity without impairing JUNO interaction. Our findings suggest a second function for IZUMO1 as a unilateral mouse gamete fusogen. |
| Volume | 222(2) |
| Published | 2023-2-6 |
| DOI | 10.1083/jcb.202207147 |
| PII | 213693 |
| PMID | 36394541 |
| PMC | PMC9671554 |
| MeSH | Animals Cell Fusion Immunoglobulins* / metabolism Male Membrane Proteins* / metabolism Mice Receptors, Cell Surface* / metabolism Semen / metabolism Sperm-Ovum Interactions* Spermatozoa / metabolism |
| IF | 8.811 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 71 |
| Altmetric score changes over past 6months | 1.0 |
| Resource | |
| Human and Animal Cells | BHK-21(RCB1423) |