| RRC ID |
73106
|
| Author |
Brukman NG, Nakajima KP, Valansi C, Flyak K, Li X, Higashiyama T, Podbilewicz B.
|
| Title |
A novel function for the sperm adhesion protein IZUMO1 in cell-cell fusion.
|
| Journal |
J Cell Biol
|
| Abstract |
Mammalian sperm-egg adhesion depends on the trans-interaction between the sperm-specific type I glycoprotein IZUMO1 and its oocyte-specific GPI-anchored receptor JUNO. However, the mechanisms and proteins (fusogens) that mediate the following step of gamete fusion remain unknown. Using live imaging and content mixing assays in a heterologous system and structure-guided mutagenesis, we unveil an unexpected function for IZUMO1 in cell-to-cell fusion. We show that IZUMO1 alone is sufficient to induce fusion, and that this ability is retained in a mutant unable to bind JUNO. On the other hand, a triple mutation in exposed aromatic residues prevents this fusogenic activity without impairing JUNO interaction. Our findings suggest a second function for IZUMO1 as a unilateral mouse gamete fusogen.
|
| Volume |
222(2)
|
| Published |
2023-2-6
|
| DOI |
10.1083/jcb.202207147
|
| PII |
213693
|
| PMID |
36394541
|
| PMC |
PMC9671554
|
| MeSH |
Animals
Cell Fusion
Immunoglobulins* / metabolism
Male
Membrane Proteins* / metabolism
Mice
Receptors, Cell Surface* / metabolism
Semen / metabolism
Sperm-Ovum Interactions*
Spermatozoa / metabolism
|
| IF |
8.811
|
| Resource |
| Human and Animal Cells |
BHK-21(RCB1423) |
