Reference - Detail
| RRC ID | 73126 |
|---|---|
| Author | Mizuta H, Takakusaki A, Suzuki T, Otake K, Dohmae N, Simizu S. |
| Title | C-mannosylation regulates stabilization of RAMP1 protein and RAMP1-mediated cell migration. |
| Journal | FEBS J |
| Abstract |
C-mannosylation is a unique type of protein glycosylation via C-C linkage between an α-mannose and a tryptophan residue. This modification has been identified in about 30 proteins and regulates several functions, such as protein secretion and intracellular localization, as well as protein stability. About half of C-mannosylated proteins are categorized as proteins containing thrombospondin type 1 repeat domain or type I cytokine receptors. To evaluate whether C-mannosylation broadly affects protein functions regardless of protein domain or family, we have sought to identify other types of C-mannosylated protein and analyse their functions. In this study, we focused on receptor activity modifying protein 1, which neither contains thrombospondin type 1 repeat domain nor belongs to the type I cytokine receptors. Our mass spectrometry analysis demonstrated that RAMP1 is C-mannosylated at Trp56 . It has been shown that RAMP1 transports to the plasma membrane after dimerization with calcitonin receptor-like receptor and is important for ligand-dependent downstream signalling activation. Our results showed that C-mannosylation has no effect on this transport activity. On the other hand, C-mannosylation did enhance protein stability and cell migration activity. Our data may provide new insight into both C-mannosylation research and novel RAMP1 analysis. |
| Volume | 290(1) |
| Pages | 196-208 |
| Published | 2023-1-1 |
| DOI | 10.1111/febs.16592 |
| PMID | 35942636 |
| MeSH | Cell Membrane Cell Movement Glycosylation Receptors, Cytokine* Thrombospondins* |
| IF | 4.392 |
| Altmetric score |
オルトメトリクス指標項目
|
| The most frequently cited source | X(Twitter) |
| Total number of mentions | 5 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | CSII-CMV-MCS-IRES2-Bsd (RDB04385) Genome Network Project Human cDNA Clone IRAL005C15 (HGY082063) |
| Human and Animal Cells | 293T(RCB2202) |