RRC ID 73229
Author Hasegawa A, Kuroda M, Wang Y, Akaki S, Asaumi J, Shibuya K, Kawasaki S, Okumura Y, Kanazawa S, Hiraki Y.
Title pEYFP-Nuc vector is a useful tool for three-dimensional and time-lapse observation of nuclear morphology of Jurkat cells during apoptosis.
Journal Int J Mol Med
Abstract Using a pEYFP-Nuc vector, which contains nucleic acid sequences of a nuclear localization signal, we established a Jurkat-YN cell line that expressed enhanced yellow fluorescent protein (EYFP) in the nucleus. We observed three-dimensional and time-lapse changes in nuclear morphology of Jurkat-YN cells during Fas-induced apoptosis using a confocal laser scanning microscope. The nuclear forms visualized by EYFP were almost equal in quality to those visualized by SYTO59, a nucleic acid stain for living cells. Three-dimensional deformities in the nuclear form were observed during apoptosis before chromatin condensation became apparent, indicating these deformities are characteristic morphological changes of the early stage of apoptosis. In conclusion, the pEYFP-Nuc vector is a useful tool in the time-lapse observation of nuclear morphology of living cells during apoptosis.
Volume 13(2)
Pages 235-42
Published 2004-2-1
PMID 14719129
MeSH Antibodies, Monoclonal / immunology Apoptosis / immunology Apoptosis / physiology* Cell Nucleus / immunology Cell Nucleus / physiology* Genes, Reporter* Genetic Vectors* Humans Jurkat Cells / cytology* Microscopy, Confocal Microscopy, Fluorescence Time Factors fas Receptor / immunology
IF 3.098
Human and Animal Cells Jurkat(RCB0806)