RRC ID 73230
著者 Sugawara T, Nomura E, Nakajima A, Sakuragi N.
タイトル Characterization of binding between SF-1 and Sp1: predominant interaction of SF-1 with the N-terminal region of Sp1.
ジャーナル J Endocrinol Invest
Abstract The transcription factor specificity protein 1 (Sp1) binds to GC boxes and interacts with many transcription factors to regulate gene expression. Steroidogenic factor-1 (SF-1) is an orphan nuclear receptor and plays a major role in regulation of the human steroidogenic acute regulatory (StAR) gene. We demonstrated that there is interaction between SF-1 and Sp1 on the human StAR promoter. In the present study, we examined the mechanism of the interaction between Sp1 and SF-1 on the human StAR gene promoter. Results of glutathione S-transferase (GST) pull-down assays and a mammalian two-hybrid assay showed that SF-1 interacted with Sp1 through the N-terminal domains of Sp1. Results of electrophoretic mobility shift assays using nuclear extracts showed that Sp1 is associated with SF-1-DNA complex formation. The density of SF-1-DNA complex was much greater when recombinant Sp1 was added to the incubation mixture. These results suggest that Sp1 interacts with SF-1 and that Sp1 enhances SF-1-DNA complex formation to regulate human StAR transcription.
巻・号 27(2)
ページ 133-41
公開日 2004-2-1
DOI 10.1007/BF03346258
PII 5404
PMID 15129808
MeSH Animals DNA-Binding Proteins / genetics DNA-Binding Proteins / metabolism* Electrophoretic Mobility Shift Assay Fushi Tarazu Transcription Factors Gene Expression Regulation Glutathione Transferase / metabolism Humans Luciferases / metabolism Mice Phosphoproteins / metabolism Sp1 Transcription Factor / genetics Sp1 Transcription Factor / metabolism* Steroidogenic Factor 1 Transcription Factors / genetics Transcription Factors / metabolism* Tumor Cells, Cultured
リソース情報
ヒト・動物細胞 Y1(RCB0533)