| RRC ID |
73277
|
| 著者 |
Fujie N, Ito M, Kishida M, Hirata Y, Kondo A, Tanaka T.
|
| タイトル |
Metabolic engineering of Schizosaccharomyces pombe for itaconic acid production.
|
| ジャーナル |
J Biotechnol
|
| Abstract |
The economical production of value-added chemicals from renewable biomass is a promising aspect of producing a sustainable economy. Itaconic acid (IA) is a high value-added compound that is expected to be an alternative to petroleum-based chemicals. In this study, we developed a metabolic engineering strategy for the large-scale production of IA from glucose using the fission yeast Schizosaccharomyces pombe. Heterologous expression of the cis-aconitic acid decarboxylase (CAD) gene from Aspergillus terreus, which encodes cis-aconitate decarboxylase in the cytosol, led to the production of 0.132 g/L of IA. We demonstrated that mitochondrial localization of CAD enhanced the production of IA. To prevent the leakage of carbon flux from the TCA cycle, we generated a strain in which the endogenous malate exporter, citrate lyase, and citrate transporter genes were disrupted. A titer of 1.110 g/L of IA was obtained from a culture of this strain started with 50 g/L of glucose. By culturing the multiple mutant strain at increased cell density, we succeeded in enhancing the IA production to 1.555 g/L. The metabolic engineering strategies presented in this study have the potential to improve the titer of the biosynthesis of derivatives of intermediates of the TCA cycle.
|
| 巻・号 |
358
|
| ページ |
111-117
|
| 公開日 |
2022-11-10
|
| DOI |
10.1016/j.jbiotec.2022.09.006
|
| PII |
S0168-1656(22)00215-2
|
| PMID |
36122598
|
| MeSH |
Aconitic Acid / metabolism
Carboxy-Lyases* / genetics
Carboxy-Lyases* / metabolism
Glucose / metabolism
Malates
Metabolic Engineering / methods
Petroleum*
Schizosaccharomyces* / genetics
Schizosaccharomyces* / metabolism
Succinates / metabolism
|
| IF |
3.503
|
| リソース情報 |
| 遺伝子材料 |
pDUAL-FFH61 (RDB06538) |
