Reference - Detail
| RRC ID | 73286 |
|---|---|
| Author | Yokoo H, Misawa T, Kato T, Tanaka M, Demizu Y, Oba M. |
| Title | Development of delivery carriers for plasmid DNA by conjugation of a helical template to oligoarginine. |
| Journal | Bioorg Med Chem |
| Abstract |
Arginine (Arg)-rich peptides can penetrate the cell membrane and deliver nucleic acid-based therapeutics into cells. In this study, a helical template designed with a repeating sequence composed of two l-leucines (l-Leu) and a 2-aminoisobutyric acid (Aib) (l-Leu-l-Leu-Aib) was conjugated to nona-arginine on either the C- or N- terminus, designated as Block 1 and Block 2. Each terminal modification induced helical structure formation and improved the physicochemical properties of peptide/plasmid DNA (pDNA) complexes, resulting in efficient intracellular pDNA delivery. The introduction of a helical template may be effective for the endosomal escape of pDNA and pDNA release from complexes in cells. These results emphasized the potency of a helical template for the development of novel cell-penetrating peptides for pDNA delivery. |
| Volume | 72 |
| Pages | 116997 |
| Published | 2022-10-15 |
| DOI | 10.1016/j.bmc.2022.116997 |
| PII | S0968-0896(22)00390-X |
| PMID | 36088811 |
| MeSH | Arginine / chemistry Cell-Penetrating Peptides* / chemistry DNA / genetics Leucine Nucleic Acids* Peptides Plasmids |
| IF | 3.073 |
| Altmetric score |
オルトメトリクス指標項目
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| The most frequently cited source | X(Twitter) |
| Total number of mentions | 1 |
| Altmetric score changes over past 6months | 0.0 |
| Resource | |
| DNA material | pCAcc-Luc+ (RDB02443) |