RRC ID 73323
Author Aikawa S, Susa T, Sato T, Kitahara K, Kato T, Kato Y.
Title Transcriptional activity of the 5' upstream region of the porcine glycoprotein hormone alpha subunit gene.
Journal J Reprod Dev
Abstract Gene expression of the porcine glycoprotein hormone alpha subunit (p-alphaGSU) was examined in LbetaT2 cells, which were established from the anterior pituitary lobe of the immortalized transgenic mouse and produce alphaGSU, and in CHO cells cloned from Chinese hamster ovaries. Expression of the reporter gene fused with p-alphaGSU gene upstream in LbetaT2 cells showed that the distal regions -540/-240 and -798/-541 are important for the activation of gene expression. In contrast, the transcriptional activity of the distal region of p-alphaGSU gene was repressed in CHO cells. The region -540/-240 contains an adequate enhancer, called pituitary glycoprotein hormone basal element, whereas the region -798/-541 has no distinguished element. Transfection of the expression vector containing cDNA of a pan-pituitary activator, Ptx1, whose putative binding sites are present scatted in the distal region of the p-alphaGSU gene, revealed unexpectedly that this factor significantly suppressed the expression of p-alphaGSU gene in LbetaT2 cells, indicating that Ptx1 is unrelated to the upregulation in the region -798/-541. Thus, this study demonstrated for the first time that the distal region -798/-541of the p-alphaGSU gene is indispensable for prominent expression of this gene in which an as yet unidentified factor may participate.
Volume 51(1)
Pages 117-21
Published 2005-2-1
DOI 10.1262/jrd.51.117
PII JST.JSTAGE/jrd/51.117
PMID 15750303
MeSH Animals Binding Sites CHO Cells Cricetinae DNA, Complementary / metabolism DNA-Binding Proteins / metabolism Gene Expression Regulation Genes, Reporter Genetic Vectors Glycoprotein Hormones, alpha Subunit / biosynthesis* Glycoprotein Hormones, alpha Subunit / genetics* Glycoproteins / metabolism Homeodomain Proteins / metabolism Paired Box Transcription Factors Pituitary Gland / metabolism Promoter Regions, Genetic Swine Time Factors Transcription Factors / metabolism Transcription, Genetic* Transfection
IF 1.735
Human and Animal Cells CHO