論文 - 詳細
| RRC ID | 73345 |
|---|---|
| 著者 | Motani K, Saito-Tarashima N, Nishino K, Yamauchi S, Minakawa N, Kosako H. |
| タイトル | The Golgi-resident protein ACBD3 concentrates STING at ER-Golgi contact sites to drive export from the ER. |
| ジャーナル | Cell Rep |
| Abstract |
STING, an endoplasmic reticulum (ER)-resident receptor for cyclic di-nucleotides (CDNs), is essential for innate immune responses. Upon CDN binding, STING moves from the ER to the Golgi, where it activates downstream type-I interferon (IFN) signaling. General cargo proteins exit from the ER via concentration at ER exit sites. However, the mechanism of STING concentration is poorly understood. Here, we visualize the ER exit sites of STING by blocking its transport at low temperature or by live-cell imaging with the cell-permeable ligand bis-pivSATE-2'F-c-di-dAMP, which we have developed. After ligand binding, STING forms punctate foci at non-canonical ER exit sites. Unbiased proteomic screens and super-resolution microscopy show that the Golgi-resident protein ACBD3/GCP60 recognizes and concentrates ligand-bound STING at specialized ER-Golgi contact sites. Depletion of ACBD3 impairs STING ER-to-Golgi trafficking and type-I IFN responses. Our results identify the ACBD3-mediated non-canonical cargo concentration system that drives the ER exit of STING. |
| 巻・号 | 41(12) |
| ページ | 111868 |
| 公開日 | 2022-12-20 |
| DOI | 10.1016/j.celrep.2022.111868 |
| PII | S2211-1247(22)01764-8 |
| PMID | 36543137 |
| MeSH | Endoplasmic Reticulum / metabolism Golgi Apparatus / metabolism Interferon Type I* / metabolism Ligands Membrane Proteins / metabolism Protein Transport / physiology Proteomics* |
| IF | 8.109 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 30 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | RAW 264(RCB0535) |