論文 - 詳細
| RRC ID | 73351 |
|---|---|
| 著者 | Kuzuhara T. |
| タイトル | Preparation of Recombinant PA Endonuclease Domain Protein of Influenza A Virus and Its Application for Glycobiology Research. |
| ジャーナル | Methods Mol Biol |
| Abstract |
The endonuclease activity of influenza A virus RNA polymerase allows the digestion of host mRNA. The PA endonuclease domain could be a target of anti-influenza A virus drugs such as glycoconjugates. To test this activity, purified viral PA endonuclease domain protein is necessary. Here, we describe a method for the expression and purification of recombinant influenza A virus PA endonuclease domain protein, and a PA endonuclease assay to test glycoconjugates for potential inhibitory activity. Using influenza A virus PA cDNA as a template, DNA from the open reading frame of the PA endonuclease domain protein was amplified with PCR and cloned into an expression vector. Six His-tagged PA endonuclease domain proteins were expressed in Escherichia coli and purified with Ni2+-agarose resin and imidazole using an ion-exchange column. Using the recombinant PA endonuclease domain protein, an endonuclease assay was performed. |
| 巻・号 | 2556 |
| ページ | 69-78 |
| 公開日 | 2022-1-1 |
| DOI | 10.1007/978-1-0716-2635-1_7 |
| PMID | 36175628 |
| MeSH | DNA, Complementary DNA-Directed RNA Polymerases Endonucleases / genetics Escherichia coli / genetics Glycomics* Imidazoles Influenza A virus* / genetics Sepharose |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pBMSA-PA (RDB01971) |