論文 - 詳細
| RRC ID | 73352 |
|---|---|
| 著者 | Hino N, Matsuda K, Jikko Y, Maryu G, Sakai K, Imamura R, Tsukiji S, Aoki K, Terai K, Hirashima T, Trepat X, Matsuda M. |
| タイトル | A feedback loop between lamellipodial extension and HGF-ERK signaling specifies leader cells during collective cell migration. |
| ジャーナル | Dev Cell |
| Abstract |
Upon the initiation of collective cell migration, the cells at the free edge are specified as leader cells; however, the mechanism underlying the leader cell specification remains elusive. Here, we show that lamellipodial extension after the release from mechanical confinement causes sustained extracellular signal-regulated kinase (ERK) activation and underlies the leader cell specification. Live-imaging of Madin-Darby canine kidney (MDCK) cells and mouse epidermis through the use of Förster resonance energy transfer (FRET)-based biosensors showed that leader cells exhibit sustained ERK activation in a hepatocyte growth factor (HGF)-dependent manner. Meanwhile, follower cells exhibit oscillatory ERK activation waves in an epidermal growth factor (EGF) signaling-dependent manner. Lamellipodial extension at the free edge increases the cellular sensitivity to HGF. The HGF-dependent ERK activation, in turn, promotes lamellipodial extension, thereby forming a positive feedback loop between cell extension and ERK activation and specifying the cells at the free edge as the leader cells. Our findings show that the integration of physical and biochemical cues underlies the leader cell specification during collective cell migration. |
| 巻・号 | 57(19) |
| ページ | 2290-2304.e7 |
| 公開日 | 2022-10-10 |
| DOI | 10.1016/j.devcel.2022.09.003 |
| PII | S1534-5807(22)00633-5 |
| PMID | 36174555 |
| MeSH | Animals Cell Movement / physiology Dogs Epidermal Growth Factor / pharmacology Extracellular Signal-Regulated MAP Kinases* / metabolism Feedback Hepatocyte Growth Factor* Mice |
| IF | 10.092 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
|
| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 135 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 遺伝子材料 | pCMV-VSV-G-RSV-Rev (RDB04393) |