論文 - 詳細
| RRC ID | 73377 |
|---|---|
| 著者 | Nakajima K, Shen Z, Miura M, Nakabayashi H, Kawahara M. |
| タイトル | Sequential control of myeloid cell proliferation and differentiation by cytokine receptor-based chimeric antigen receptors. |
| ジャーナル | PLoS One |
| Abstract |
As chimeric antigen receptor (CAR)-T cell therapy has been recently applied in clinics, controlling the fate of blood cells is increasingly important for curing blood disorders. In this study, we aim to construct proliferation-inducing and differentiation-inducing CARs (piCAR and diCAR) with two different antigen specificities and express them simultaneously on the cell surface. Since the two antigens are non-cross-reactive and exclusively activate piCAR or diCAR, sequential induction from cell proliferation to differentiation could be controlled by switching the antigens added in the culture medium. To demonstrate this notion, a murine myeloid progenitor cell line 32Dcl3, which proliferates in an IL-3-dependent manner and differentiates into granulocytes when cultured in the presence of G-CSF, is chosen as a model. To mimic the cell fate control of 32Dcl3 cells, IL-3R-based piCAR and G-CSFR-based diCAR are rationally designed and co-expressed in 32Dcl3 cells to evaluate the proliferation- and differentiation-inducing functions. Consequently, the sequential induction from proliferation to differentiation with switching the cytokine from IL-3 to G-CSF is successfully replaced by switching the antigen from one to another in the CARs-co-expressing cells. Thus, piCAR and diCAR may become a platform technology for sequentially controlling proliferation and differentiation of various cell types that need to be produced in cell and gene therapies. |
| 巻・号 | 17(12) |
| ページ | e0279409 |
| 公開日 | 2022-1-1 |
| DOI | 10.1371/journal.pone.0279409 |
| PII | PONE-D-22-21208 |
| PMID | 36574389 |
| PMC | PMC9794043 |
| MeSH | Animals Cell Differentiation Cell Proliferation Granulocyte Colony-Stimulating Factor / pharmacology Interleukin-3 / metabolism Mice Myeloid Progenitor Cells / metabolism Receptors, Chimeric Antigen* / genetics Receptors, Cytokine |
| IF | 2.74 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| ヒト・動物細胞 | 32Dcl3(RCB1377) |