RRC ID 73399
Author Yoshida K, Saiga H.
Title Repression of Rx gene on the left side of the sensory vesicle by Nodal signaling is crucial for right-sided formation of the ocellus photoreceptor in the development of Ciona intestinalis.
Journal Dev Biol
Abstract Nodal signaling plays an essential role in the establishment of left-right asymmetry in various animals. However, it is largely unknown how Nodal signaling is involved in the establishment of the left-right asymmetric morphology. In this study, the role of Nodal signaling in the left-right asymmetric ocellus formation in the ascidian, Ciona intestinalis was dealt with. During the development of C. intestinalis, the ocellus pigment cell forms on the midline and moves to the right side of the midline. Then, the photoreceptor cells form on the right side of the sensory vesicle (SV). Ci-Nodal is expressed on the left side of the SV in the developing tail bud embryo. When Nodal signaling is inhibited, the ocellus pigment cell form but remain on the midline, and expression of marker genes of the ocellus photoreceptor cells is ectopically detected on the left side as well as on the right side of the SV in the larva. Furthermore, Ci-Rx, which is essential for the ocellus differentiation, turns out to be negatively regulated by the Nodal signaling on the left side of the SV, even though it is required for the right-sided photoreceptor formation. These results indicate that Nodal signaling controls the left-right asymmetric ocellus formation in the development of C. intestinalis.
Volume 354(1)
Pages 144-50
Published 2011-6-1
DOI 10.1016/j.ydbio.2011.03.006
PII S0012-1606(11)00155-2
PMID 21402066
MeSH Animals Body Patterning / genetics Ciona intestinalis / embryology Ciona intestinalis / genetics* Ciona intestinalis / metabolism Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Female Gene Expression Regulation, Developmental Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism Homeodomain Proteins / genetics* Homeodomain Proteins / metabolism In Situ Hybridization Male Microscopy, Confocal Nervous System / embryology Nervous System / metabolism* Nodal Protein / genetics* Nodal Protein / metabolism Photoreceptor Cells, Invertebrate / metabolism* Reverse Transcriptase Polymerase Chain Reaction Signal Transduction
IF 2.896
C.intestinalis / (O.japonicus) Wild C. int