論文 - 詳細
| RRC ID | 73401 |
|---|---|
| 著者 | Yamada S, Ueno N, Satoh N, Takahashi H. |
| タイトル | Ciona intestinalis Noto4 contains a phosphotyrosine interaction domain and is involved in the midline intercalation of notochord cells. |
| ジャーナル | Int J Dev Biol |
| Abstract |
Brachyury plays a pivotal role in the notochord formation in ascidian embryos. Ciona intestinalis Noto4 (Ci-Noto4) was isolated as a gene downstream of Ci-Bra. This gene encodes a 307 amino-acid protein with a C-terminal phosphotyrosine interaction domain (PTB/PID). Expression of Ci-Noto4 commences at the neural plate stage and is specific to notochord cells. Suppression of Ci-Noto4 levels with specific antisense morpholino oligonucleotides resulted in the formation of two rows of notochord cells owing to a lack of midline intercalation between the bilateral populations of progenitor cells. In contrast, overexpression of Ci-Noto4 by injection of a Ci-Bra(promoter):Ci-Noto4-EGFP construct into fertilized eggs disrupted the localization of notochord cells. Ci-Noto4 overexpression did not affect cellular differentiation in the notochord, muscle, mesenchyme, or nervous system. Analysis of Ci-Noto4 regions that are responsible for its function suggested significant roles for the PTB/PID and a central region, an area with no obvious sequence similarity to other known proteins. These results suggested that PTB/PID-containing Ci-Noto4 is essential for midline intercalation of notochord cells in chordate embryos. |
| 巻・号 | 55(1) |
| ページ | 11-8 |
| 公開日 | 2011-1-1 |
| DOI | 10.1387/ijdb.092981sy |
| PII | 092981sy |
| PMID | 20979028 |
| MeSH | Amino Acid Sequence Animals Binding Sites / genetics Ciona intestinalis / embryology Ciona intestinalis / genetics* Ciona intestinalis / metabolism Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Fetal Proteins / genetics Gene Expression Regulation, Developmental Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism In Situ Hybridization Molecular Sequence Data Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Notochord / cytology Notochord / embryology Notochord / metabolism* Oligonucleotides, Antisense / genetics Phosphotyrosine / metabolism Promoter Regions, Genetic / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism T-Box Domain Proteins / genetics |
| IF | 1.105 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 各媒体での言及数の合計 | 0 |
| リソース情報 | |
| カタユウレイボヤ・(ニッポンウミシダ) | Wild C. int |