RRC ID 73401
Author Yamada S, Ueno N, Satoh N, Takahashi H.
Title Ciona intestinalis Noto4 contains a phosphotyrosine interaction domain and is involved in the midline intercalation of notochord cells.
Journal Int J Dev Biol
Abstract Brachyury plays a pivotal role in the notochord formation in ascidian embryos. Ciona intestinalis Noto4 (Ci-Noto4) was isolated as a gene downstream of Ci-Bra. This gene encodes a 307 amino-acid protein with a C-terminal phosphotyrosine interaction domain (PTB/PID). Expression of Ci-Noto4 commences at the neural plate stage and is specific to notochord cells. Suppression of Ci-Noto4 levels with specific antisense morpholino oligonucleotides resulted in the formation of two rows of notochord cells owing to a lack of midline intercalation between the bilateral populations of progenitor cells. In contrast, overexpression of Ci-Noto4 by injection of a Ci-Bra(promoter):Ci-Noto4-EGFP construct into fertilized eggs disrupted the localization of notochord cells. Ci-Noto4 overexpression did not affect cellular differentiation in the notochord, muscle, mesenchyme, or nervous system. Analysis of Ci-Noto4 regions that are responsible for its function suggested significant roles for the PTB/PID and a central region, an area with no obvious sequence similarity to other known proteins. These results suggested that PTB/PID-containing Ci-Noto4 is essential for midline intercalation of notochord cells in chordate embryos.
Volume 55(1)
Pages 11-8
Published 2011-1-1
DOI 10.1387/ijdb.092981sy
PII 092981sy
PMID 20979028
MeSH Amino Acid Sequence Animals Binding Sites / genetics Ciona intestinalis / embryology Ciona intestinalis / genetics* Ciona intestinalis / metabolism Embryo, Nonmammalian / embryology Embryo, Nonmammalian / metabolism Fetal Proteins / genetics Gene Expression Regulation, Developmental Green Fluorescent Proteins / genetics Green Fluorescent Proteins / metabolism In Situ Hybridization Molecular Sequence Data Nerve Tissue Proteins / genetics* Nerve Tissue Proteins / metabolism Notochord / cytology Notochord / embryology Notochord / metabolism* Oligonucleotides, Antisense / genetics Phosphotyrosine / metabolism Promoter Regions, Genetic / genetics Recombinant Fusion Proteins / genetics Recombinant Fusion Proteins / metabolism T-Box Domain Proteins / genetics
IF 1.105
C.intestinalis / (O.japonicus) Wild C. int