論文 - 詳細
| RRC ID | 73520 |
|---|---|
| 著者 | Wang Z, Wei H, Wu Z, Zhang X, Sun Y, Gao L, Zhang W, Su YQ, Zhang M. |
| タイトル | The oocyte cumulus complex regulates mouse sperm migration in the oviduct. |
| ジャーナル | Commun Biol |
| Abstract |
As the time of ovulation draws near, mouse spermatozoa move out of the isthmic reservoir, which is a prerequisite for fertilization. However, the molecular mechanism remains unclear. The present study revealed that mouse cumulus cells of oocytes-cumulus complexes (OCCs) expressed transforming growth factor-β ligand 1 (TGFB1), whereas ampullary epithelial cells expressed the TGF-β receptors, TGFBR1 and TGFBR2, and all were upregulated by luteinizing hormone (LH)/human chorionic gonadotropin (hCG). OCCs and TGFB1 increased natriuretic peptide type C (NPPC) expression in cultured ampullae via TGF-β signaling, and NPPC treatment promoted spermatozoa moving out of the isthmic reservoir of the preovulatory oviducts. Deletion of Tgfb1 in cumulus cells and Tgfbr2 in ampullary epithelial cells blocked OCC-induced NPPC expression and spermatozoa moving out of the isthmic reservoir, resulting in compromised fertilization and fertility. Oocyte-derived paracrine factors were required for promoting cumulus cell expression of TGFB1. Therefore, oocyte-dependent and cumulus cell-derived TGFB1 promotes the expression of NPPC in oviductal ampulla, which is critical for sperm migration in the oviduct and subsequent fertilization. |
| 巻・号 | 5(1) |
| ページ | 1327 |
| 公開日 | 2022-12-3 |
| DOI | 10.1038/s42003-022-04287-8 |
| PII | 10.1038/s42003-022-04287-8 |
| PMID | 36463362 |
| PMC | PMC9719508 |
| MeSH | Animals Female Fertilization / genetics Fertilization / physiology Male Mice Natriuretic Peptide, C-Type* / genetics Natriuretic Peptide, C-Type* / metabolism Oocytes* / metabolism Oocytes* / physiology Oviducts* / metabolism Oviducts* / physiology Ovulation* / genetics Ovulation* / metabolism Receptor, Transforming Growth Factor-beta Type II / genetics Receptor, Transforming Growth Factor-beta Type II / metabolism Semen Sperm Transport* / genetics Sperm Transport* / physiology Spermatozoa* / metabolism |
| IF | 4.165 |
| オルトメトリクス指標 |
オルトメトリクス指標項目
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| 最多言及媒体 | X(Twitter) |
| 各媒体での言及数の合計 | 1 |
| 過去6か月間でのオルトメトリクス指標の変動値 | 0.0 |
| リソース情報 | |
| 実験動物マウス | RBRC03743 |