| RRC ID |
73520
|
| 著者 |
Wang Z, Wei H, Wu Z, Zhang X, Sun Y, Gao L, Zhang W, Su YQ, Zhang M.
|
| タイトル |
The oocyte cumulus complex regulates mouse sperm migration in the oviduct.
|
| ジャーナル |
Commun Biol
|
| Abstract |
As the time of ovulation draws near, mouse spermatozoa move out of the isthmic reservoir, which is a prerequisite for fertilization. However, the molecular mechanism remains unclear. The present study revealed that mouse cumulus cells of oocytes-cumulus complexes (OCCs) expressed transforming growth factor-β ligand 1 (TGFB1), whereas ampullary epithelial cells expressed the TGF-β receptors, TGFBR1 and TGFBR2, and all were upregulated by luteinizing hormone (LH)/human chorionic gonadotropin (hCG). OCCs and TGFB1 increased natriuretic peptide type C (NPPC) expression in cultured ampullae via TGF-β signaling, and NPPC treatment promoted spermatozoa moving out of the isthmic reservoir of the preovulatory oviducts. Deletion of Tgfb1 in cumulus cells and Tgfbr2 in ampullary epithelial cells blocked OCC-induced NPPC expression and spermatozoa moving out of the isthmic reservoir, resulting in compromised fertilization and fertility. Oocyte-derived paracrine factors were required for promoting cumulus cell expression of TGFB1. Therefore, oocyte-dependent and cumulus cell-derived TGFB1 promotes the expression of NPPC in oviductal ampulla, which is critical for sperm migration in the oviduct and subsequent fertilization.
|
| 巻・号 |
5(1)
|
| ページ |
1327
|
| 公開日 |
2022-12-3
|
| DOI |
10.1038/s42003-022-04287-8
|
| PII |
10.1038/s42003-022-04287-8
|
| PMID |
36463362
|
| PMC |
PMC9719508
|
| MeSH |
Animals
Female
Fertilization / genetics
Fertilization / physiology
Male
Mice
Natriuretic Peptide, C-Type* / genetics
Natriuretic Peptide, C-Type* / metabolism
Oocytes* / metabolism
Oocytes* / physiology
Oviducts* / metabolism
Oviducts* / physiology
Ovulation* / genetics
Ovulation* / metabolism
Receptor, Transforming Growth Factor-beta Type II / genetics
Receptor, Transforming Growth Factor-beta Type II / metabolism
Semen
Sperm Transport* / genetics
Sperm Transport* / physiology
Spermatozoa* / metabolism
|
| IF |
4.165
|
| リソース情報 |
| 実験動物マウス |
RBRC03743 |
