RRC ID 73524
Author Yamada S, Masuno H, Kagechika H, Tanatani A, Kanda Y.
Title A Novel Lithocholic Acid Derivative Upregulates Detoxification-Related Genes in Human Induced Pluripotent Stem Cell-Derived Intestinal Organoids.
Journal Biol Pharm Bull
Abstract Vitamin D is a fat-soluble micronutrient that plays essential roles in a range of biological processes, including cell proliferation, inflammation, and metabolism. In this study, we investigated the effects of a novel synthetic lithocholic acid derivative with vitamin D activity (Dcha-20) on pharmacokinetic gene expression in human induced pluripotent stem cell-derived intestinal organoids. Compared with vitamin D3 treatment, Dcha-20 was found to upregulate the expression and enzyme activity of the drug-metabolizing enzyme CYP3A4, an indicator of intestinal functional maturation. In addition, Dcha-20 specifically increased expression levels of the xenobiotic detoxification enzyme UGT1A and excretion transporter MRP2. These results suggest that Dcha-20 promotes activity of the intrinsic defense system of the intestinal epithelium.
Volume 45(11)
Pages 1720-1724
Published 2022-1-1
DOI 10.1248/bpb.b22-00492
PMID 36328508
MeSH Cell Differentiation Humans Induced Pluripotent Stem Cells* / metabolism Intestinal Mucosa / metabolism Lithocholic Acid / metabolism Lithocholic Acid / pharmacology Organoids Vitamin D / metabolism Vitamin D / pharmacology
IF 1.863
Human and Animal Cells 253G1(HPS0002)