RRC ID 73564
著者 Toprak Ö, Topuz B, Monsef YA, Oto Ç, Orhan K, Karakeçili A.
タイトル BMP-6 carrying metal organic framework-embedded in bioresorbable electrospun fibers for enhanced bone regeneration.
ジャーナル Mater Sci Eng C Mater Biol Appl
Abstract Biomolecule carrier structures have attracted substantial interest owing to their potential utilizations in the field of bone tissue engineering. In this study, MOF-embedded electrospun fiber scaffold for the controlled release of BMP-6 was developed for the first time, to enrich bone regeneration efficacy. The scaffolds were achieved by first, one-pot rapid crystallization of BMP-6 encapsulated ZIF-8 nanocrystals-as a novel carrier for growth factor molecules- and then electrospinning of the blending solution composed of poly (ε-caprolactone) and BMP-6 encapsulated ZIF-8 nanocrystals. BMP-6 molecule encapsulation efficiency for ZIF-8 nanocrystals was calculated as 98%. The in-vitro studies showed that, the bioactivity of BMP-6 was preserved and the release lasted up to 30 days. The release kinetics fitted the Korsmeyer-Peppas model exhibiting a pseudo-Fickian behavior. The in-vitro osteogenesis studies revealed the superior effect of sustained release of BMP-6 towards osteogenic differentiation of MC3T3-E1 pre-osteoblasts. In-vivo studies also revealed that the sustained slow release of BMP-6 was responsible for the generation of well-mineralized, new bone formation in a rat cranial defect. Our results proved that; MOF-carriers embedded in electrospun scaffolds can be used as an effective platform for bone regeneration in bone tissue engineering applications. The proposed approach can easily be adapted for various growth factor molecules for different tissue engineering applications.
巻・号 120
ページ 111738
公開日 2021-1-1
DOI 10.1016/j.msec.2020.111738
PII S0928-4931(20)33657-2
PMID 33545881
MeSH Absorbable Implants Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Protein 6 Bone Regeneration Cell Differentiation Mesenchymal Stem Cells* Metal-Organic Frameworks* Osteogenesis Rats Tissue Engineering Tissue Scaffolds
IF 5.88
リソース情報
ヒト・動物細胞 MC3T3-E1(RCB1126)