Reference - Detail
RRC ID | 73611 |
---|---|
Author | Kim IS, Song GY, Kim DH, Cho SH, Yun CY, Lee JS. |
Title | Effect of (E)-2-(3,4-dimethoxyphenyl)-4-oxo-4H-chromen-7-yl-3-(3,4-dimethoxyphenyl) acrylate on the development of atopic dermatitis-like lesions. |
Journal | Life Sci |
Abstract |
AIMS:In this study, we synthesized a novel chemical, (E)-2-(3,4-dimethoxyphenyl)-4-oxo-4H-chromen-7-yl-3-(3,4-dimethoxyphenyl) acrylate (CSH) and investigated the effect of CSH on atopic dermatitis (AD) by evaluating the anti-inflammatory effect of CSH on immune cells in vitro and on atopic dermatitis-like lesions in vivo. MAIN METHODS:Human monocytic THP-1 cells and human eosinophilic EoL-1 cells were treated with house dust mite extract in the absence and presence of CSH. Nc/Nga mice were sensitized to 2,4-dinitrochlorobenzne (DNCB) for 5 weeks and then orally and dorsally administered with CSH or dexamethasone for 3 weeks. KEY FINDINGS:CSH inhibited the secretion of monocyte chemotactic protein-1 (MCP-1), interleukin (IL)-6 and IL-8 due to house dust mite extract in THP-1 cells. CSH also suppressed the secretion of MCP-1 and IL-8 in EoL-1 cells. In vivo, the skin severity score decreased after CSH treatment as compared to the control group. CSH suppressed the inflammatory cell infiltration into the dermis and thickened the epidermis. CSH reduced serum IgE level as compared to the control group. The levels of IL-4, IL-5, IL-13 and eotaxin in mouse splenocytes increased after treatment with concanavalin A and the increase of the cytokines was decreased by pre-treatment with CSH. The inhibitory effects of CSH on atopic lesions of DNCB-treated Nc/Nga mice were similar to those of dexamethasone, despite differing degrees depending on results evaluated in this study. SIGNIFICANCE:These results may contribute to the development of a therapeutic drug for the treatment of AD. |
Volume | 91(9-10) |
Pages | 338-44 |
Published | 2012-9-24 |
DOI | 10.1016/j.lfs.2012.07.021 |
PII | S0024-3205(12)00383-9 |
PMID | 22871385 |
MeSH | Animals Anti-Inflammatory Agents / administration & dosage Anti-Inflammatory Agents / pharmacology* Cell Line Chemokine CCL2 / immunology Chemokine CCL2 / metabolism Concanavalin A / toxicity Coumaric Acids / administration & dosage Coumaric Acids / pharmacology* Coumarins / administration & dosage Coumarins / pharmacology* Cytokines / immunology Cytokines / metabolism Dermatitis, Atopic / drug therapy* Dermatitis, Atopic / immunology Dermatitis, Atopic / pathology Dexamethasone / administration & dosage Dexamethasone / pharmacology Disease Models, Animal Eosinophils / drug effects* Eosinophils / immunology Female Humans Immunoglobulin E / blood Interleukin-6 / immunology Interleukin-6 / metabolism Interleukin-8 / immunology Interleukin-8 / metabolism Mice Monocytes / drug effects* Monocytes / immunology Pyroglyphidae / immunology Severity of Illness Index Spleen / cytology Spleen / drug effects |
IF | 3.647 |
Resource | |
Human and Animal Cells | EoL-1 cell(RCB0641) |